文献类型：期刊文献

英文题名：Significantly Accelerated Osteoblast Cell Growth on TiO2/SrHA Composite Mediated by Phenolic Compounds (BHM) from Hippocamp us kuda Bleeler

作者：Yuan, Qiong[1]; He, Lei[2]; Qian, Zhong-Ji[2,3]; Zhou, Chunxia[1,3,4]; Hong, Pengzhi[1,3,4]; Wang, Zhe[5]; Wang, Yi[6]; Sun, Shengli[2]; Li, Chengyong[2,3,4]

机构：[1] College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, 524088, China; [2] School of Chemistry and Environment, Guangdong Ocean University, Zhanjiang, 524088, China; [3] Shenzhen Institute of Guangdong Ocean University, Shenzhen, 518108, China; [4] Guangdong Modern Agricultural Science and Technology Innovation Center, Zhanjiang, 524088, China; [5] Center for Biomedical Materials and Interfaces, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China; [6] Department of Applied Biology and Chemical Technology, Hong Kong Polytechnic University, Special Administrative Region, Kowloon, Hong Kong, 999077, Hong Kong

年份：2018

卷号：10

期号：36

起止页码：30214

外文期刊名：ACS Applied Materials and Interfaces

收录：EI(收录号：20183505757308)、Scopus(收录号：2-s2.0-85052280986)

语种：英文

外文关键词：Phenols - Targeted drug delivery - Hydroxyapatite - Transcription - Scaffolds (biology) - Strontium - Substrates - Controlled drug delivery - Bone - Cell adhesion - Cell proliferation - Nanotubes - Phosphatases - Titanium dioxide - Strontium compounds

外文摘要：The microstructure of hydroxyapatite is known to influence cellular behavior, can be used as a substrate for osteoblast growth, and exploited as a drug-release platform. However, easy delamination and self-decomposition of hydroxyapatite caused by poor adhesion with substrates are the main problems currently. In this paper, we successfully fabricated titanium dioxide/strontium-doped hydroxyapatite (TiO2/SrHA) composite scaffolds by self-generated strontium-substituted hydroxyapatite microspheres in TiO2 nanotubes. Moreover, the active compound 1-(5-bromo-2-hydroxy-methoxyphenyl)-ethanone (BHM) from Seahorse (Hippocampus kuda Bleeler) was loaded in this scaffold, and the controlled release kinetics of BHM was studied. It was found that in the first 5 h, the release concentration and time of BHM had a good linear relationship, and the correlation coefficient reached 0.98. TiO2/SrHA/BHM composites exhibited favorable cytocompatibility at a given concentration of BHM (20 μmol/L). Compared to pure SrHA, TiO2 nanotubes, and traditional TiO2/SrHA composites, superior cytocompatibility (cell adhesion and proliferation) of MC3T3-E1 was obtained on TiO2/SrHA/BHM composites. The expression levels of osteogenic marker genes such as alkaline phosphatase, osteopontin, osteocalcin, runt-related transcription factor 2, and collagen I are also upregulated to varying degrees. This TiO2/SrHA composite scaffold-mediated phenolic compound BHM could be applied in bone tissue repair. ? 2018 American Chemical Society.