文献类型：期刊文献

中文题名：一株海洋源菌细菌素CAMT2生物学特性研究

英文题名：Characterization of bacterioncin CAMT2 produced by marine-source strain

作者：吴焕婷[1];邓木兰[1];焦冬冬[1];秦伟灵[1];房志家[1];孙力军[1];刘颖[1]

机构：[1]广东海洋大学食品科技学院,广东湛江524088

年份：2017

卷号：38

期号：15

起止页码：107

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD、、北大核心2014、CSCD_E2017_2018、北大核心、CSCD

基金：广东海洋大学"海之帆--起航计划"大学生科技创新项目(hzfqhjhzrkx2015b06);广东省科技计划项目(2014A020217018;2016A020222014;2014B020205006;2014B020204005);广东省高等学校创新强校项目(GDOU2013050205;GDOU2013050203)

语种：中文

中文关键词：解淀粉芽孢杆菌;细菌素;生物特性

外文关键词：Bacillus amyloliquefaciens ; bacteriocin ; biological characterization

中文摘要：本文以解淀粉芽孢杆菌(Bacillus amyloliquefaciens)ZJHD3-06发酵产生的细菌素CAMT2为研究对象,以金黄色葡萄球菌、单增李斯特菌、大肠杆菌、副溶血性弧菌及芽孢杆菌作为指示菌,利用牛津杯扩散法测定不同温度、p H、蛋白酶、有机溶剂对CAMT2抗菌活性的影响,并对CAMT2的乳化特性进行了研究。结果表明:CAMT2具有很好的热稳定性,在100℃处理20 min抑菌活性仍保留60%以上。p H在6~10时抑菌活性具有很强的稳定性,但p H在2、4时抗菌活性有一定的降低,活性降低率在9.4%~51.9%。CAMT2对4种蛋白酶有不同程度的敏感性,对二甲基甲酰胺和二氯甲烷两种有机溶剂有较高的耐受力。细菌素CAMT2对供试菌均有抑菌作用,表现出一定的抗菌谱,CAMT2所具有的这些特性为其进一步开发与应用奠定了一定的理论基础。

外文摘要：Bacteriocin CAMT2, produced by fermentation of Bacillus amyloliquefaciens ZJHD3- 06, was regard as research subject, and Staphylococcus aureus, Listeria monocytogenes, Escherichia coli, Vibrio parahemolyticus, Bacillus subtilis were used as indicator bacteria.Anti- bacterial activity of bacteriocin CAMT2 treated by different temperatures, pH, proteases, and organic solvents were determined with oxford cup method. Besides, the effects of several hydrophobic substanceson emulsification property of CAMT2 were investigated. The results showed that CAMT2 had relatively high thermo- stability that after being treated under 100 ℃ for 20 min, and above 60% of its anti-bacterial activity was still retained.Within the range of pH6~ 10, the antimicrobial activity still kept stability, while antimicrobial activity had certain decrease, and active loss rates ranged from 9.4% to 51.9% in pH2 and 4. The sensitivities of CAMT2 to 4 different proteases were different. High tolerance to dimethylformamide and dichloromethane was shown.The result also showed the anti-bacterial activity of bacteriocin CAMT2 on indicator bacteria,and thus its certain spectrum of anti-bacterial action.Those properties of CAMT2 laid a foundation of theory for further development and application.