文献类型：期刊文献

中文题名：近江牡蛎糖胺聚糖的酶解提取及其抗肿瘤活性研究

英文题名：STUDY ON ENZYMATIC PREPARATION OF GLYCOSAMINOGLYCAN WITH ANTI-TUMOR ACTIVITY FROM CRASSOSTREARIVULARIS CROULD

作者：胡雪琼[1];吴红棉[1];刘芷筠[1];钱庆华[1];范秀萍[1]

机构：[1]广东海洋大学食品科技学院,广东湛江524025

年份：2009

卷号：30

期号：7

起止页码：3

中文期刊名：食品研究与开发

外文期刊名：Food Research and Development

收录：CSTPCD、、北大核心2008、北大核心

基金：广东海洋大学自然科学基金团队项目(0612258)

语种：中文

中文关键词：近江牡蛎;糖胺聚糖;酶解;抗肿瘤活性

外文关键词：Crassostrearivularis Crould; glycosaminoglycan; enzymatic hydrolysis; anti-tumor activity

中文摘要：探讨酶解法提取近江牡蛎糖胺聚糖(SG1)的条件,结果表明:先用枯草杆菌中性蛋白酶1.0%(质量分数),pH7.0,50℃酶解5h,再加胰蛋白酶1.0%(质量分数),pH7.8,50℃酶解5h效果最佳。粗提物SG1的得率为2.40%,总糖胺聚糖含量为13.6%,总糖含量为61.3%。采用四氮唑蓝还原法(MTT法)研究粗提物SG1对人宫颈癌细胞(Hela细胞)的体外抗肿瘤活性,结果显示:500μg/mL剂量组与600μg/mL剂量组48h时的抑制率分别为43.1%(P<0.05),55.8%(P<0.05),近江牡蛎糖胺聚糖粗提物SG1具有较明显的抗肿瘤活性,存在一定的量效关系。

外文摘要：The extraction conditions of crude glycosaminoglycan （SG1） by enzymatic hydrolysis from Crassostrearivularis Crould were studied in this paper. The results showed that the optimum conditions were： slurry of Crassostrearivularis Crould adding neutral protease of Bacillus subtilis, enzyme dosage 1.0 %（w/v）, pH 7.0, temperature 50 ℃, enzymatic hydrolysis time 5 h, then adding trypsin enzyme with dosage 1.0 %（w/v）, pH 7.8, temperature 50 ℃ and time 5 h. The extraction ratio of the crude glycosaminoglycan （SG1）, the total content of glycosaminoglycan and its total sugar respectively were 2.40 %, 13.6 % and 61.3 %. Then the anti-tumor activity in vitro to cell Hela of crude glycosaminoglycan by MTT assay was investgated. It turned out that the inhibition rates of the dose groups （500 μg/mL and 600 μg/mL） respectively were 43.1% （P〈0.05）and 55.8 % （P〈0.05）.The crude glycosaminoglycan（SG1） from Crassostrearivularis Crould had marked anti-tumor function which was concentration-dependent.