文献类型：期刊文献

中文题名：鲑降钙素对虹鳟鳞组织miRNA表达的影响

英文题名：Effects of salmon calcitonin on the expression of miRNA in Oncorhynchus mykiss scales

作者：周启苓[1];马骞[1,2];王刘永[1];毛非凡[1];杨二军[1];陈刚[1,2]

机构：[1]广东海洋大学水产学院,广东湛江524025;[2]广东海洋大学,南方海洋科学与工程广东省实验室(湛江),广东湛江524025

年份：2024

卷号：48

期号：3

起止页码：44

中文期刊名：水产学报

外文期刊名：Journal of Fisheries of China

收录：北大核心2023、CSTPCD、、Scopus、CSCD2023_2024、北大核心、CSCD

基金：国家自然科学基金(31772828);广东海洋大学科研启动经费(R19022)。

语种：中文

中文关键词：虹鳟;miRNA;鲑降钙素;鳞;钙代谢;骨代谢

外文关键词：Oncorhynchus mykiss;miRNA;salmon calcitonin;scales;calcium metabolism;bone matebolism

中文摘要：miRNA作为一类非编码小RNA分子,在脊椎动物骨代谢的分子调控网络中占有重要地位。本研究旨在探究钙调节因子对硬骨鱼类骨组织miRNA表达水平的影响。采用鲑降钙素(salmon calcitonin, sCT)对虹鳟幼鱼进行腹腔注射,并在注射24 h后采集鳞片,利用高通量测序技术和生物信息学方法对其中的miRNA表达谱进行分析。注射组及对照组样品miRNA测序分别获得14 051 631和15 816 147条高质量miRNA序列(18~26 nt),并分别从中鉴定出568和592种成熟miRNA。注射sCT后的虹鳟鳞片中共筛选出24个差异表达miRNA (DEMs,其中9个表达上调,15个表达下调)。随后,从中随机选取8个miRNA进行实时荧光定量PCR (qRT-PCR)检测,其检测结果与高通量测序结果一致。GO注释和富集分析结果显示,DEMs的预测靶基因主要被注释在金属离子结合、钙离子结合、G蛋白偶联受体信号通路、Wnt信号通路和经典Wnt信号通路等功能上,靶基因主要在NF-kappaB输入细胞核的负调节、IL-1β分泌的负调节和TGF-β结合等功能上显著富集。KEGG富集分析结果显示,DEMs的靶基因显著富集在Toll样受体和雌激素等信号通路中。基于上述分析结果,本研究筛选出omy-miR-29a-5p、omy-miR-30d-5p、omy-mir-125b-2-p3、omy-miR-138、omy-miR-199b-5p和omy-miR-200b等多个可能参与虹鳟骨代谢调控过程的miRNA。本实验筛选出的差异miRNA可为硬骨鱼类骨代谢调控机制研究提供素材。

外文摘要：As a class of small non-coding RNA molecules,miRNA plays an important role in the molecular regu-latory network of vertebrate bone metabolism.Normally,bone metabolism is referred to the modeling and remod-eling processes in regulating calcium homeostasis,and scale tissue of fish is an ideal model for the study of bone metabolism due to its abundant quantity and easy acquisition.Salmon calcitonin(sCT)is a small peptide hormone isolated from the gills of salmonidae,which can inhibit the activity of osteoclasts to regulate calcium homeostasis of fish.In order to explore the regulatory mechanism of teleost bone metabolism,intraperitoneal injection of sCT was performed in Oncorhynchus mykiss juveniles and scales were collected 24 h after the injection.High-through-put sequencing technology and bioinformatics methods were performed to evaluate the effect of sCT on the miRNA expression profiles in scales.The results showed that 14051631 and 15816147 high quality miRNA sequences(18-26 nt)were obtained from the samples of the injection group(IG)and control group(CG),respect-ively,and 568 and 592 mature miRNA were identified in IG and CG scales,respectively.A total of 24 differen-tially expressed miRNAs(DEMs)including nine up regulated and 15 down regulated DEMs were identified.Eight miRNAs were randomly selected for quantitative real-time PCR(qRT-PCR)detection,and results were consistent with the RNA-Seq results.GO annotation and enrichment analysis revealed that the predicted target genes of DEMs were mainly annotated in the functions of metal ion binding,calcium ion binding,G protein coupled receptor signaling pathway,Wnt signaling pathway and classical Wnt signaling pathway;these genes were enriched in the negative regulation of NF-kappaB input into the nucleus,the negative regulation of interleukin-1β(IL-1β)secretion and the binding of transforming growth factorβ(TGF-β).KEGG enrichment analysis showed that the predicted target genes of DEMs were significantly enriched in toll-like receptor and estrogen-related sig-naling pathways.A total of six miRNA(omy-miR-29a-5p,omy-miR-30d-5p,omy-miR-125b-2-p3,omy-miR-138,omy-miR-199b-5p and omy-miR-200b)were identified,which could provide materials for future studies on the molecular mechanism of bone metabolism in teleosts.