文献类型：期刊文献

英文题名：Biotransformation enzyme activities and phase I metabolites analysis in Litopenaeus vannamei following intramuscular administration of T-2 toxin

作者：Deng, Yijia[1];Wang, Yaling[1];Sun, Lijun[1];Lu, Pengli[1];Wang, Rundong[1];Ye, Lin[1];Xu, Defeng[1];Ye, Riying[1];Liu, Ying[1];Bi, Siyuan[2];Gooneratne, Ravi[3]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Zhanjiang, Peoples R China;[2]Shenzhen Bioeasy Biotechnol Co, Shenzhen, Peoples R China;[3]Lincoln Univ, Dept Wine Food & Mol Biosci, Ctr Food Res & Innovat, Lincoln, New Zealand

年份：2018

卷号：41

期号：1

起止页码：113

外文期刊名：DRUG AND CHEMICAL TOXICOLOGY

收录：SCI-EXPANDED(收录号：WOS:000417762600015)、、WOS

基金：This work was supported by the National Natural Science Foundation of China (NSFC) [No. 31171634 and No. 31371777], the Science, Technology Planning Project of Guangdong Province [No. 2014B020205006], the Guangdong Oceanic & Fishery Administration Project [Guangdong Fiscal Government on Agriculture [2015] No. 115], a Project of the Enhancing Innovation School of Guangdong Ocean University [No. 2013050205 and No. 2014050203 and No. GDOU 2013050312] and the Shenzhen Technology Research & Development Foundation Project [GCZX20140509163151273].

语种：英文

外文关键词：Litopenaeus vannamei; T-2 toxin; metabolizing enzymes; carboxylesterase; metabolite analysis

外文摘要：T-2 toxin (T-2) is a type-A trichothecene produced by Fusarium that causes toxicity to animals. T-2 contamination of grain-based aquatic feed is a concern for the industries related to edible aquatic crustacean species such as the shrimp industry because it can lead to serious food safety issues. T-2, its metabolites, and selected phase I (EROD, CarE) and phase II (GST, UGT, SULT) detoxification enzymes in hemolymph and tissues were monitored at 0, 5, 10 15, 30, 45, and 60 min following T-2 intramuscular administration (3 mg/kg bw) in shrimp (Litopenaeus vannamei). Marked increases of EROD activity in hepatopancreas and CarE activity in hemolymph, gill, hepatopancreas and intestine were observed followed by increases in phase II enzymes (GST, UGT, SULT) in hepatopancreas, hemolymph, intestine and gill, which remained elevated for an extended period. Time-dependent decrease in shrimp tissue T-2 concentration was observed. HT-2 increased up to 15 min. Most other T-2 metabolites were detected but not T-2 tetraol. Enzyme responses on exposure to T-2 were tissue-specific and time-dependent. Detection results indicated that HT-2 may not be the only important metabolite in aquatic crustacean species. Further investigation into T-2 metabolite toxicity is needed to fully understand the food safety issues related to T-2.