文献类型：期刊文献

英文题名：Dietary administration of Bacillus (B. licheniformis and B. subtilis) and isomaltooligosaccharide influences the intestinal microflora, immunological parameters and resistance against Vibrio alginolyticus in shrimp, Penaeus japonicus (Decapoda: Penaeidae)

作者：Zhang, Qin[2];Tan, Beiping[1];Mai, Kangsen[2];Zhang, Wenbing[2];Ma, Hongming[2];Ai, Qinghui[2];Wang, Xiaojie[2];Liufu, Zhiguo[2]

机构：[1]Guangdong Ocean Univ, Lab Aquat Econ Anim Nutr & Feed, Zhanjiang 524288, Peoples R China;[2]Ocean Univ China, Minist Educ, Key Lab Mariculture, Qingdao, Peoples R China

年份：2011

卷号：42

期号：7

起止页码：943

外文期刊名：AQUACULTURE RESEARCH

收录：SCI-EXPANDED(收录号：WOS:000292310400005)、、Scopus(收录号：2-s2.0-79958762383)、WOS

基金：This study was supported by grant numbers 2003AA622060, 2006AA100313 from the National 863 High-Tech Program and No. 30871928 from the National Natural Science Foundation of China (NSFC). We are grateful to X. R. Chen for feed preparation and shrimp rearing. Thanks are also to F. P. Tan for his assistance in the study.

语种：英文

外文关键词：Bacillus; oligosaccharide; Penaeus japonicus; immunity; Vibrio alginolyticus

外文摘要：The experiment was conducted to investigate the effects of oral administration of probiotics (Bacillus licheniformis and Bacillus subtilis) in combination with prebiotic isomaltooligosaccharide (IMO) on the intestinal microflora and immunological parameters of Penaeus japonicus and its resistence against Vibrio alginolyticus. A basal diet was supplemented with 0% probiotics and prebiotic (control), 10(8) colony forming unit (CFU) g(-1) B. licheniformis and 0.2% IMO (T1), 10(8) CFUg(-1) B. subtilis and 0.2% IMO (T2), 10(8) CFUg(-1) B. licheniformis in combination with 108 CFUg(-1) B. subtilis and 0.2% IMO (T3). The results showed that the total bacterial counts significantly increased (P<0.05) and Vibrio counts significantly decreased (P<0.05) in the intestine of shrimp with supplementation of dietary synbiotics. Shrimp fed the diet with both Bacillus probiotics and IMO (T3) produced significantly higher immune parameters (phenoloxidase activity, lysozyme activity and nitric oxide synthase activity and superoxide dismutase activity) than the control group (P<0.05). Significant lower cumulative mortality after challenge with V. alginolyticus was also observed in shrimp fed diet with both Bacillus probiotics and IMO (T3) than that in the control group (P<0.05). The results indicated that the oral administration of probiotics (B. licheniformis and B. subtilis) and prebiotic IMO has positive effects on bacterial flora in P. japonicus gut, and can activate non-specific immunity of shrimp.