文献类型：期刊文献

英文题名：Molecular cloning and functional characterization of spexin in orange-spotted grouper (Epinephelus coioides)

作者：Li, Shuisheng[1,2,3];Liu, Qiongyu[2,3];Xiao, Ling[2,3];Chen, Huapu[1];Li, Guangli[1];Zhang, Yong[2,3];Lin, Haoran[2,3]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang City State Key Lab Marine Ecol & Enviro, Zhanjiang 524088, Peoples R China;[2]Sun Yat Sen Univ, State Key Lab Biocontrol, Inst Aquat Econ Anim, Guangzhou 510275, Guangdong, Peoples R China;[3]Sun Yat Sen Univ, Guangdong Prov Key Lab forAquat Econ Anim, Guangzhou 510275, Guangdong, Peoples R China

年份：2016

卷号：196

起止页码：85

外文期刊名：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000376705300010)、、Scopus(收录号：2-s2.0-84960914995)、WOS

基金：This study was supported by the National Natural Science Foundation of China (31261160493), the Guangdong Provincial Natural Science Foundation (2015A030313069), the Special Fund for Fisheries-Scientific Research of Guangdong Province (A201400A01, A201501A03, A201501A09 and A201408A06), Guangdong Ocean University Science Project (nos. E15181 and hdyq2015002) and Hainan International Science and Technology Cooperation Projects (no. KJHZ2015-08).

语种：英文

外文关键词：Spexin; Reproduction; Feeding control; Orange-spotted grouper

外文摘要：Spexin is a newly discovered neuropeptide in vertebrates. Comprehensive comparative studies are required to unveil its biological functions. In order to ascertain the neuroendocrine function of spexin in orange-spotted grouper, its full-length cDNA and genomic DNA sequences were cloned and analyzed. Sequence analyses showed that the spexin gene structure is composed of six exons and five introns, and the amino acids of mature peptide (spexin-14) in grouper are identical to that of other fish. Tissue expression analysis found that grouper spexin is highly expressed in the brain, liver and ovary. Real time-PCR analysis demonstrated that the hypothalamic expression of spexin declined gradually during the ovarian development, and was up-regulated by food deprivation. Intraperitoneal administration of spexin-14 peptides to grouper significantly elevated the mRNA levels of proopiomelanocortin (pomc) and suppressed the orexin expression in the hypothalamus, but could not change the hypothalamic expression of gonadotropin releasing hormone 1 (gnrh1). Both in vivo and in vitro administration of spexin could not significantly influence the expression of follicle-stimulating hormone beta (fsh beta) and luteinizing hormone beta (lh beta) in the pituitary with the exception of an inhibition of gh expression. Our data suggested that the spexin has a significant role in the regulation of energy metabolism and food intake in orange-spotted grouper. (C) 2016 Elsevier Inc. All rights reserved.