文献类型：期刊文献

英文题名：Immunostimulatory effects of artificial feed supplemented with a Chinese herbal mixture on Oreochromis niloticus against Aeromonas hydrophila

作者：Tang, Jufen[1,2,3];Cai, Jia[1,2,3];Liu, Ran[1,2,3];Wang, Jiamin[1,2,3];Lu, Yishan[1,2,3];Wu, Zaohe[2,3,4];Jian, Jichang[1,2,3]

机构：[1]Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524088, Guangdong, Peoples R China;[2]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524088, Peoples R China;[3]Guangdong Key Lab Control Dis Aquat Econ Anim, Zhanjiang 524088, Peoples R China;[4]Zhongkai Univ Agr & Engn, Guangzhou 510225, Guangdong, Peoples R China

年份：2014

卷号：39

期号：2

起止页码：401

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000339773000036)、、WOS

基金：The authors wish to thank all the laboratory members' suggestion on this manuscript. This work was supported by Science and Technology Planning Project of Guangdong Province (2007A020200006-10, 2008A020100014) and Special Fund for Agro-scientific Research in the Public Interest (201203085).

语种：英文

外文关键词：Oreochromis niloticus; Chinese herbal mixture; Immune response; Disease resistance; Aeromonas hydrophila

外文摘要：The effects of a Chinese herbal mixture (CHM) composed of astragalus, angelica, hawthorn, Licorice root and honeysuckle on immune responses and disease resistant of Nile tilapia (Oreochromis niloticus GIFT strain) were investigated in present study. Fish were fed diets containing 0 (control), 0.5%, 1.0%, 1.5% or 2.0% CHM (w/w) for 4 weeks. And series of immune parameters including lysozyme, cytokine genes TNF-alpha and IL-1 beta, superoxide dismutase (SOD), peroxidase (POD), malondialdehyde (MDA) were measured during test period. After four weeks of feeding, fish were infected with Aeromonas hydrophila and mortalities were recorded. Results of this study showed that feeding Nile tilapia with CHM-supplementation diet stimulated lysozyme activity, SOD activity and POD activity in serum, induced TNF-alpha and IL-1 beta mRNA expression in head kidney and spleen, but decreased serum MDA content. All CHM-supplemental groups showed reduced mortalities following A. hydrophila infection compared with the group fed the control diet. These results suggested that this CHM can be applied as a tilapia feed supplement to elevate fish immunity and disease resistance against A. hydrophila. (C) 2014 Elsevier Ltd. All rights reserved.