文献类型：会议论文

英文题名：Modulation of transcription of genes related to the hypothalamic-pituitary-gonadal and the hypothalamic-pituitary-adrenal axes in zebrafish (Dario rerio) embryos/larvae by androstenedione

作者：Hou, Li-ping[1];Shu, Hu[1];Lin, Le-le[1];Xu, Si-yuan[1];Wu, Yan-xia[1];Rong, Xiu-jun[1];Hu, Jun-jie[1];Song, Li-ying[1];Liang, Yan-qiu[2];Chen, Hong-xing[4];Ying, Guang-guo[3,4];Tian, Chang-en[1]

机构：[1]Guangzhou Univ, Sch Life Sci, Guangzhou 510655, Guangdong, Peoples R China;[2]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjian 524088, Peoples R China;[3]Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Guangdong, Peoples R China;[4]South China Normal Univ, Environm Res Inst, Key Lab Environm Theoret Chem, Guangzhou 510006, Guangdong, Peoples R China

会议论文集：7th Biannual ECOtoxicology MEeting (BECOME) - Managing Aquatic and Terrestrial Environments - An Ecotoxicological Perspective

会议日期：NOV 22-24, 2016

会议地点：Livorno, ITALY

语种：英文

外文关键词：Hypothalamic-pituitary-gonadal axis; Hypothalamic-pituitary-adrenal axis; Androstenodione; Zebrafish; Embryos/larvae

外文摘要：This study aimed to determine the effects of Androstenodione (AED) on the transcriptional expression of genes involved in the hypothalamic-pituitary-gonadal (HPG) and the hypothalamic-pituitary-adrenal (HPA) axes in the zebrafish embryos/larvae. Zebrafish embryos were exposed to 0, 4.0, 45.0, 487.0, and 980.0 ng/L of AED from the day of fertilization to 144 h post fertilization (hpf), during which the transcriptional profiles of key genes related to the HPG and HPA axes were examined daily using quantitative real-time PCR. The AED exposure significantly up-regulated several receptor signaling pathways and the key genes involved in various steps of the steroidogenic pathways were also affected. In addition, the AED exposure could significantly modulate the transcriptional profiles of the other target genes related to hypothalamic and pituitary hormones. The findings of this study suggest that AED, at environmentally relevant concentrations, affects the adrenal endocrine systems and the reproduction of zebrafish by interrupting the HPG and HPA axes.