文献类型：期刊文献

中文题名：海蛾水提取物对小鼠系统低度炎症的干预作用

英文题名：Intervention effect of sea moth water extract on systemic low-grade inflammation in mice

作者：温婉宁[1];王晨[1];胡连花[1];房志家[1];邓旗[1];孙力军[1]

机构：[1]广东海洋大学食品科技学院,广东省水产品加工与安全重点实验室,广东省海洋生物制品工程实验室,广东省海洋食品工程技术研究中心,水产品深加工广东普通高等学校重点实验室,广东湛江524088

年份：2024

卷号：36

期号：12

起止页码：2784

中文期刊名：浙江农业学报

外文期刊名：Acta Agriculturae Zhejiangensis

收录：北大核心2023、、CSCD_E2023_2024、北大核心、CSCD

基金：国家自然科学基金(32172215);广东省重点领域研发计划(2021B0202060001)。

语种：中文

中文关键词：系统低度炎症;辣椒素;海蛾水提取物;肠道菌群;短链脂肪酸

外文关键词：systemic low-grade inflammation;capsaicin;sea moth water extract;intestinal flora;short chain fat acids(SCFAs)

中文摘要：为了探讨不同剂量海蛾水提取物对辣椒素引起的小鼠肠道菌群紊乱诱发的系统低度炎症的干预作用,选用30只雄性C57BL/6小鼠,随机分为空白对照组、辣椒素致炎模型组、鱼油对照组,以及海蛾水提取物低、中、高剂量组(0.2、0.4、0.6 g·kg^(-1)·d^(-1)),干预7 d后,分别采用酶联免疫法、鲎试剂法测定小鼠血清中炎症因子细胞白介素-10(IL-10)、细胞白介素-1β(IL-1β)、细胞白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)和脂多糖(lipopolysaccharide,LPS)的含量;利用16S rRNA基因高通量测序和生物信息学方法考察小鼠粪便中肠道菌群组成变化;利用气相色谱质谱法(GC-MS法)测定小鼠粪便中短链脂肪酸(short chain fat acids,SCFAs)水平。结果显示,辣椒素干预后,小鼠血清中炎症因子TNF-α质量浓度为空白组的2倍左右,小鼠系统低度炎症模型建立成功。与模型组相比,海蛾水提取物干预使模型小鼠的炎症因子和LPS含量得以恢复,粪便乳杆菌属(Lactobacillus)和拟杆菌属(Bacteroides)等益生菌属丰度显著提高,寡养单胞菌属(Stenotrophoomonas)、棒状杆菌属(Corynebacterium)等有害菌属丰度显著降低,各菌属水平均基本恢复至空白组水平;总SCFAs质量分数显著提高,且趋于空白组水平;单一SCFAs如乙酸、丙酸、丁酸基本恢复至正常水平。结果证明,海蛾水提取物能调节小鼠肠道菌群紊乱,恢复小鼠肠道SCFAs质量分数,阻止LPS入血,抑制炎症反应,缓解系统低度炎症反应。

外文摘要：To investigate the effects of different doses of sea moth water extracts on systemic low-grade inflammation induced by capsaicin-induced intestinal flora disorder in mice,in this study,thirty male C57BL/6 mice were randomly divided into blank control group,capsaicin-induced inflammation model group,fish oil control group,and sea moth water extract low-dose(0.2 g·kg^(-1)·d-1),medium-dose(0.4 g·kg^(-1)·d-1)and high-dose groups(0.6 g·kg^(-1)·d-1).The contents of inflammatory cytokines interleukin-10(IL-10),interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)and lipopolysaccharide(LPS)in serum of mice were determined by enzyme-linked immunoassay and limulus reagent method,respectively.16S rRNA gene high-throughput sequencing and bioinformatics methods were used to investigate the changes of intestinal flora in mouse feces.Mass fraction of short chain fat acids(SCFAs)in mouse feces were determined by gas chromatography-mass spectrometry(GC-MS).After the intervention of capsaicin,the mass concentration of the inflammatory factor TNF-αin the serum of mice was about twice that of the blank group,and the low-grade inflammation model of the mouse system was successfully established.The results showed that compared with the model group,the contents of inflammatory factors and LPS of the model mice were restored after the intervention with water extract of sea moth;the abundance of probiotic genera in feces such as Lactobacillus and Bacteroides increased significantly,the abundance of harmful bacteria genera such as Oligotrophomonas and Corynebacterium decreased significantly,and the levels of all bacteria genera basically recovered to the level of blank group;the mass fraction of total SCFAs was significantly increased and tended to the level of blank group,single SCFAs such as acetic acid,propionic acid,butyric acid levels were basically restored to normal levels.The water extract of sea moth can regulate the intestinal flora disorder of mice,restore the intestinal SCFAs mass fraction of mice,prevent LPS from entering the blood,inhibit the inflammatory response,and alleviate the systemic low-grade inflammation.