文献类型：期刊文献

英文题名：Phthalic acid esters degradation by a novel marine bacterial strain Mycolicibacterium phocaicum RL-HY01: Characterization, metabolic pathway and bioaugmentation

作者：Ren, Lei[1,2];Wang, Guan[1,2];Huang, Yongxiang[1,2];Guo, Jianfu[1,2];Li, Chengyong[1,2];Jia, Yang[3];Chen, Sha[4];Zhou, John L.[5];Hu, Hanqiao[1,2]

机构：[1]Guangdong Ocean Univ, Shenzhen Res Inst, Shenzhen 518108, Peoples R China;[2]Guangdong Ocean Univ, Sch Chem & Environm, Coll Coastal Agr Sci, Zhanjiang 524088, Peoples R China;[3]Wenzhou Univ, Zhejiang Prov Key Lab Subtrop Water Environm & Ma, Natl & Local Joint Engn Res Ctr Ecol Treatment Te, Wenzhou 325035, Peoples R China;[4]Hunan Univ Technol, Sch Life Sci & Chem, Hunan Key Lab Biomass Fiber Funct Mat, Zhuzhou 412007, Peoples R China;[5]Univ Technol Sydney, Ctr Green Technol, 15 Broadway, Sydney, NSW 2007, Australia

年份：2021

卷号：791

外文期刊名：SCIENCE OF THE TOTAL ENVIRONMENT

收录：SCI-EXPANDED(收录号：WOS:000686012800009)、、EI(收录号：20212410498483)、Scopus(收录号：2-s2.0-85107626497)、WOS

基金：The authors acknowledge financial support from the National Natural Science Foundation of China (31800109), Project of Enhancing School With Innovation of Guangdong Ocean University (GDOU2017052604), Key-Area Research and Development Program of Guangdong Province (2020B020219004), Natural Science Foundation of Hunan Province (2019JJ50143), Scientific Research Projects of Hunan Education Department (20B187 and 20C0588), South China Sea Scholar of Guangdong Ocean University (002029002004), Young Innovative Talents Program of Guangdong (2017KQNCX088), and Program for Scientific Research start-up Funds of Guangdong Ocean University (R18013).

语种：英文

外文关键词：Marine microbe; Phthalic acid esters; Metabolic kinetics; Bioaugmentation; Mycolicibacterium phocaicum

外文摘要：Phthalic acid esters (PAEs) are one of the most widely used plasticizers and the well-studied environmental pol -lutants with endocrine disrupting properties. Investigation about PAEs in terrestrial ecosystem has been exten-sively conducted while the fate of PAEs in marine environment remains underexplored. In this study, a novel di-(2-ethylhexyl) phthalate (DEHP) degrading marine bacterial strain, Mycolicibacterium phocaicum RL-HY01, was isolated and characterized from intertidal sediments. Strain RL-HY01 could utilize a range of PAE plasticizers as sole carbon source for growth. The effects of different environmental factors on the degradation of PAEs were evaluated and the results indicated that strain RL-HY01 could efficiently degrade PAEs under a wide range of pH (5.0 to 9.0), temperature (20 degrees C to 40 degrees C) and salinity (below 10%). Specifically, when Tween-80 was added as solubilizing agent, strain RL-HY01 could rapidly degrade DEHP and achieve complete degradation of DEHP (50 mg/L) in 48 h. The kinetics of DEHP degradation by RL-HY01 were well fitted with the modified Gompertz model. The metabolic intermediates of DEHP by strain RL-HY01 were identified by ultra-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analysis and then the metabolic pathway of DEHP was deduced. DEHP was transformed into di-ethyl phthalate (DEP) via beta-oxidation and then DEP was hydrolyzed into phthalic acid (PA) by de-esterification. PA was further transformed into gentisate via salicylic acid and further utilized for cell growth. Bioaugmentation of strain RL-HY01 with marine samples was performed to evaluate its application potential and the results suggested that strain RL-HY01 could accelerate the elimination of DEHP in marine samples. The results have advanced our understanding of the fate of PAEs in marine ecosystem and identified an efficient bioremediation strategy for PAEs-polluted marine sites. (c) 2021 Elsevier B.V. All rights reserved.