文献类型：期刊文献

英文题名：Comprehensive utilization of black soldier fly (Hermetia illucens) larvae: extraction, recovery and characterization of peptide, chitin and melanin and scaling-up trial

作者：Chen, Yongkang[1,2];Guo, Yucai[1,2];He, Xuanshu[1,2];Tan, Beiping[3];Liao, Zhihong[1,2];Chen, Anqi[1,2];Gu, Xingyu[1,2];Li, Xiaomin[1,2];Chen, Xuanqi[1,2];Chen, Baoyang[1,2];Lin, Sihan[1,2];Li, Wenjie[4];Hu, Peng[5];Zhu, Xinliang[5];Zhao, Wei[1,2];Niu, Jin[1,2]

机构：[1]Sun Yat Sen Univ, State Key Lab Biocontrol, Guangdong Prov Key Lab Aquat Econ Anim, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China;[2]Sch Life Sci Sun Yat Sen Univ, Southern Marine Sci & Engn Guangdong Lab Zhuhai, Guangzhou 510275, Guangdong, Peoples R China;[3]Guangdong Ocean Univ, Coll Fisheries, Lab Aquat Nutr & Feed, Zhanjiang 524088, Peoples R China;[4]Qinghai Kunjie Environm Protect Technol Co LTD, Xining, Qinghai, Peoples R China;[5]Henan Subcrit Biotechnol Co LTD, Key Lab Subcrit Extract Anyang City, Anyang 455000, Henan, Peoples R China

年份：2025

卷号：361

外文期刊名：SEPARATION AND PURIFICATION TECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:001412504400001)、、EI(收录号：20250117623345)、Scopus(收录号：2-s2.0-85213507717)、WOS

基金：This research was supported by the National Key R&D Program of China (2023YFD2402000) , and Project of Science and Technology of Guangdong Province (2023A1515012627) , and Central Government Guides Local Funds for Science and Technology Development of Science and Technology of Qinghai Province (2023ZY015) , and Project of National Natural Science Foundation of China (32172982) , and Supported by China Agriculture Research System of MOF and MARA-48.

语种：英文

外文关键词：Black soldier fly larvae; Chitin; Melanin; Peptide; Protein recovery; Scaling-up trial

外文摘要：The nutrients, chitin and pigments resources in black soldier fly (Hermetia illucens) (BSF) recently attracts attention. However, current procedure for sequential extraction of these BSF resources in industrial scale remains not clearly established. Our study developed an extraction process for peptide, chitin and melanin based on defatted BSF (DFBSF). The results showed that enzymatic hydrolysis with alkaline protease and keratinase could reduce the DFBSF substrate by 81.05 % (dry basis). After hydrolysis, the mixture was filtered and the hydrolysate was collected and dried to yield BSF peptide (BSFP), and then melanin and chitin were extracted from the remained insect residue. The melanin extraction process was optimized as liquid-to-solid ratio of 15:1, NaOH 2.2 M, and temperature of 70 degrees C for 90 min to obtain the maximum yield. Chitin was then extracted from the solid fraction after the melanin extraction. The BSFP was characterized by 61.86 % crude protein, 54.09 % total amino acids, and 88.30 % of peptides with molecular weights (Mw) below 1000 Da. The spectral, elemental composition and HPLC properties indicated that BSF melanin mainly consists of eumelanin. The absorption bands of IR spectra at 1650, 1619, and 1551 cm- 1 indicated that BSF chitin belongs to alpha-crystalline chitin. A 50 kg scaling-up trial was conducted to validate the industrial feasibility of this process. Through this process, 50.00 kg of DFBSF was transformed into 32.24 kg BSFP, 0.85 kg melanin and 5.77 kg chitin. The establishment of BSFP, chitin and melanin extraction provided the insect farming industry with efficient conversion of biomass into high valueadded materials.