文献类型：期刊文献

英文题名：Biocompatibility of Veratric Acid-Encapsulated Chitosan/Methylcellulose Hydrogel: Biological Characterization, Osteogenic Efficiency with In Silico Molecular Modeling

作者：Durairaj, Kaliannan[1,2];Balasubramanian, Balamuralikrishnan[3];Arumugam, Vijaya Anand[4];Easwaran, Murugesh[5];Park, Sungkwon[3];Issara, Utthapon[6];Pushparaj, Karthika[7];Al-Dhabi, Naif Abdullah[8];Arasu, Mariadhas Valan[8];Liu, Wen-Chao[9];Mousavi Khaneghah, Amin[10,11]

机构：[1]Periyar Univ, Sch Life Sci, Dept Environm Sci, Salem 636011, India;[2]Wonkwang Univ, Zoonosis Res Ctr, Sch Med, Dept Infect Biol, Iksan 54538, South Korea;[3]Sejong Univ, Coll Life Sci, Dept Food Sci & Biotechnol, Seoul 05006, South Korea;[4]Bharathiar Univ, Dept Human Genet & Mol Biol, Coimbatore 641046, Tamil Nadu, India;[5]Bharathiar Univ, Dept Bioinformat, Computat Biol Lab, Coimbatore 641046, Tamil Nadu, India;[6]Rajamangala Univ Technol Thanyaburi, Fac Sci & Technol, Div Food Sci & Technol Management, Khlong Hok 12110, Thailand;[7]Avinashilingam Inst Home Sci & Higher Educ Women, Sch Biosci, Dept Zool, Coimbatore 641043, Tamil Nadu, India;[8]King Saud Univ, Coll Sci, Dept Bot & Microbiol, POB 2455, Riyadh 11451, Saudi Arabia;[9]Guangdong Ocean Univ, Coll Agr, Dept Anim Sci, Zhanjiang 524088, Guangdong, Peoples R China;[10]Prof Waclaw Dabrowski Inst Agr, Food Biotechnol State Res Inst, Dept Fruit & Vegetable Prod Technol, 36 Rakowiecka St, PL-02532 Warsaw, Poland;[11]Azerbaijan State Oil & Ind Univ, Dept Technol Chem, Baku, Azerbaijan

年份：2023

卷号：195

期号：7

起止页码：4429

外文期刊名：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000922920600002)、、EI(收录号：20230513461406)、Scopus(收录号：2-s2.0-85146851950)、WOS

基金：All the authors were grateful to the authorities of their institutes~and~Researchers Supporting Project number (RSP-2023-R20), King Saud University, Riyadh, Saudi Arabia.

语种：英文

外文关键词：Bone regeneration; Chitosan-methylcellulose; Hydrogel; Molecular docking; Scaffold; Veratric acid

外文摘要：The limitations of graft material, and surgical sites for autografts in bone defects treatment have become a significant challenge in bone tissue engineering. Phytocompounds markedly affect bone metabolism by activating the osteogenic signaling pathways. The present study investigated the biocompatibility of the bio-composite thermo-responsive hydrogels consisting of chitosan (CS), and methylcellulose (MC) encapsulated with veratric acid (VA) as a restorative agent for bone defect treatment. The spectroscopy analyses confirmed the formation of CS/MC hydrogels and VA encapsulated CS/MC hydrogels (CS/MC-VA). Molecular analysis of the CS-specific MC decamer unit with VA complex exhibited a stable integration in the system. Further, Runx2 (runt-related transcription factor 2) was found in the docking mechanism with VA, indicating a high binding affinity towards the functional site of the Runx2 protein. The formulated CS/MC-VA hydrogels exhibited biocompatibility with the mouse mesenchymal stem cells, while VA promoted osteogenic differentiation in the stem cells, which was verified by calcium phosphate deposition through the von Kossa staining. The study results suggest that CS/MC-VA could be a potential therapeutic alternative source for bone regeneration.