文献类型：期刊文献

中文题名：热处理对负载叶黄素的罗非鱼分离蛋白乳液稳定性和体外消化的影响

英文题名：Effect of heat treatment on stability and in vitro digestion of lutein loaded tilapia protein isolate

作者：陈艾霖[1];洪鹏志[1,2];宋春勇[1];冯瑞[1];李婷[1];周春霞[1,2];林琼妮[1]

机构：[1]广东海洋大学食品科技学院,广东省水产品加工与安全重点实验室,广东省海洋食品工程技术研究中心,广东湛江524088;[2]南方海洋科学与工程广东省实验室(湛江),广东湛江524088

年份：2021

卷号：47

期号：16

起止页码：173

中文期刊名：食品与发酵工业

外文期刊名：Food and Fermentation Industries

收录：CSTPCD、、CSCD2021_2022、北大核心、CSCD、北大核心2020

基金：广东省科技计划项目(2017A020208067);广东省现代农业产业技术体系创新团队建设项目(2019KJ150)。

语种：中文

中文关键词：罗非鱼分离蛋白;叶黄素;乳液;稳定性;体外消化

外文关键词：tilapia protein isolate;lutein;emulsion;stability;in vitro digestion

中文摘要：叶黄素是一种具有多种生物活性的类胡萝卜素,因高疏水性和低稳定性,其应用受到限制。为提升叶黄素的稳定性和生物利用率,以罗非鱼分离蛋白(tilapia protein isolate,TPI)作为乳化剂,高压均质制备负载叶黄素(200μg/mL)的TPI乳液,探讨热处理(60、70、80、90℃,30 min)对乳液的稳定性和体外消化的影响。结果表明,热处理后,叶黄素乳液的粒径减小(P<0.05),电位绝对值增大(P<0.05),4℃下贮藏28 d无分层现象,且热处理温度低于80℃不会造成叶黄素降解。热处理能增加液滴的表面静电荷和乳液黏度,有利于抵抗液滴之间的聚集,乳液稳定性增加。其中,70℃加热30 min后叶黄素乳液的稳定性最好,乳液粒径减小至(490.33±8.42)nm,游离脂肪酸释放量达(94.22±2.67)%,叶黄素生物利用率为(35.69±2.06)%。TPI乳液能够作为疏水活性物质的载体,且热处理能明显提高乳液的稳定性和叶黄素的生物利用率,为叶黄素的应用提供新思路。

外文摘要：Lutein is a kind of carotenoid with many biological activities.Its application is limited due to its high hydrophobicity and low stability.In order to improve the stability and bioavailability of lutein,tilapia protein isolate(TPI)emulsion loading with lutein(200μg/mL)was prepared by high pressure homogenization using tilapia protein isolate(TPI)as an emulsifying agent.The effects of heat treatment(60,70,80,90℃,30 min)on the stability and digestion of the emulsion were investigated.The results showed that with heat treatment,the particle size of lutein emulsion decreased(P<0.05),however,the absolute potential increased(P<0.05).And there was no delamination at 4℃for 28 d.Moreover,the degradation of lutein was not caused when the heat treatment temperature was lower than 80℃.Besides,the heat treatment could increase the surface static charge of the droplet and the viscosity of the emulsion,which was beneficial to resist the aggregation of the droplet and increase the stability of the emulsion.After heat treatment at 70℃for 30 min,lutein emulsion had the best stability,the particle size of the emulsion was reduced to(490.33±8.42)nm.And the release amount of free fatty acids was(94.22±2.67)%and the bioavailability of lutein was(35.69±2.06)%respectively.TPI emulsion can be used as a carrier of hydrophobic active substances.And heat treatment can significantly improve the stability of the emulsion and the bioavailability of lutein,which provides new insights for the application of lutein.