文献类型：期刊文献

英文题名：Effects of temperature and fish oil supplementation on ovarian development and foxl2 mRNA expression in spotted scat Scatophagus argus

作者：Li, G. -L.[1,2];Zhang, M. -Z.[1,2];Deng, S. -P.[1,2];Chen, H. -P.[1,2];Zhu, C. -H.[1,2]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Key Lab Aquaculture South China Sea Aquat Econ An, Guangdong Higher Educ Inst, Zhanjiang 524088, Peoples R China

年份：2015

卷号：86

期号：1

起止页码：248

外文期刊名：JOURNAL OF FISH BIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000347359200017)、、Scopus(收录号：2-s2.0-84920503604)、WOS

基金：This study was supported by the National Natural Science Foundation of China (number 31272640). Special thanks are extended to Zhuhai Longsheng Fry Cultivation Base (Zhuhai, Guangdong, China) for rearing the fish. We gratefully acknowledge Y. -X. Tao (Auburn University, Auburn, AL, U.S.A.) for expert editing of the manuscript.

语种：英文

外文关键词：environmental factors; gene expression; oestradiol-17; sex steroid hormones

外文摘要：In this study, the complete foxl2 complementary (c)DNA sequence was isolated by simple modular-architecture research tool (SMART)er rapid amplification of cDNA ends (RACE). Two year-old female spotted scat, Scatophagus argus, were reared at different temperatures (23, 26 and 29 degrees C) for 6 weeks, or fed with different concentrations of dietary fish oil (0, 2 or 6%) for 8 weeks. Ovarian development, serum oestradiol-17 (E-2) levels, as well as ovarian foxl2 expression were measured. At the end of experiment, ovarian foxl2 messenger (m)RNA expression in fish reared at 23 and 26 degrees C was significantly higher than that in fish reared at 29 degrees C, and that in 2 and 6% fish oil groups was also significantly higher than that in control group (P < 005). Serum E-2 levels exhibited the same trend with foxl2 mRNA expression in temperature treatment groups and fish oil fed groups. There was a significant positive correlation between stage of oocytes and foxl2 expressions. Results showed that from 23 to 29 degrees C, the optimal temperature for ovarian development in S. argus was 23-26 degrees C, and 6% fish oil supplementation could effectively promote ovarian development. Optimal temperature and fish oil supplement might increase ovarian foxl2 mRNA expressions to promote ovarian development in S. argus.