文献类型：期刊文献

中文题名：不同磷肥水平对甘蔗脯氨酸合成积累及关键基因表达的效应

英文题名：Effects of different phosphorus levels on proline biosynthesis and accumulation and key gene expression in sugarcane

作者：杨善[1];叶昌辉[1];莫俊杰[1];黄兴[1];杨杰文[1];周鸿凯[1]

机构：[1]广东海洋大学农学院,广东湛江524088

年份：2015

卷号：36

期号：6

起止页码：68

中文期刊名：华南农业大学学报

外文期刊名：Journal of South China Agricultural University

收录：CSTPCD、、北大核心2014、CSCD2015_2016、Scopus、北大核心、CSCD

基金：国家自然科学基金(41073059);广东省科技计划项目(2013B020301005;2013B020301008;2010B020302008);广东省良种培育和引进专项(201201148)

语种：中文

中文关键词：甘蔗;磷肥用量;脯氨酸含量;P5CS;δ-OAT;基因表达;干旱胁迫

外文关键词：sugarcane ; phosphorus application rate ; proline content ; 6-OAT; P5CS ; gene expression ; drought stress

中文摘要：【目的】探索不同磷肥量对甘蔗脯氨酸的合成积累影响,以阐明磷肥对甘蔗脯氨酸合成积累及其耐旱性的作用效应.【方法】以甘蔗栽培种粤糖55为研究材料,用桶栽试验法,在正常水分和干旱胁迫条件下,测定4个磷肥施用水平的甘蔗植株内P5CS、δ-OAT基因表达和酶活性、以及游离脯氨酸、叶绿素含量等生理生化指标.【结果和结论】无论是在正常供水条件下还是干旱胁迫下,甘蔗P5CS、δ-OAT基因的表达及酶活性、游离脯氨酸含量均受到N、P、K配比的影响.当施用过磷酸钙为900 kg·hm-2时,正常水分条件下,P5CS、δ-OAT酶活性均处于较低的水平,植株游离脯氨酸含量最低;干旱胁迫下,P5CS酶活性最高,δ-OAT酶活性亦处于较高水平,植株游离脯氨酸含量增加到最大.基于本研究的结果,湛江砖红壤蔗地的最佳N、P、K肥搭配方案是尿素、过磷酸钙、氯化钾的用量分别为918、900、750 kg·hm-2.相关、偏相关及通径分析的结果表明,干旱胁迫下植株中P5CS对游离脯氨酸的合成积累贡献显著大于δ-OAT;干旱胁迫下甘蔗合成积累游离脯氨酸的途径是以谷氨酸途径(Glu→Pro)为主,鸟氨酸途径(Orn→Pro)为辅.

外文摘要：【Objective】Different phosphorus（P）fertilizer rates were applied to clarify the effect of P fertilizer on proline biosynthesis and accumulation and drought-resistance in sugarcane.【Method】Sugarcane variety YT-55 was used as the plant material for barrel experiment. Under the normal and drought conditions, four P levels were designed. The physiological and biochemical indexes such as the expressions of P5CS and δ-OAT genes, enzyme activities and the contents of free proline and chlorophyll were determined.【Result and conclusion】The expressions of P5CS and δ-OAT genes,enzyme activities and the free proline content were affected by the ratios of N, P and K fertilizers under the normal and drought conditions. When the P level was 900 kg·hm^-2, both P5CS and δ-OAT activities were relatively low, and the free proline content was the lowest in the plants under the normal water condition. The P5CS activity was the highest; the δ-OAT activity was also at a high level; and the free proline content was up to the maximum amount under drought stress. Based on the results, the optimum levels of N, P and K fertilizers are 918 kg·hm^-2 urea, 900 kg·hm^-2 calcium superphosphate and 750 kg·hm^-2 potassium chloride in Zhanjiang lateritic soil. The results of correlation, partial correlation and path analysis indicated that the P5CS was more effective than δ-OAT in free proline biosynthesis and accumulation of sugarcane under drought stress. The main pathway of free proline biosynthesis and accumulation is glutamic acid pathway （Glu→Pro） supplemented by ornithine pathway （Orn→ Pro） under drought stress.