文献类型：期刊文献

英文题名：Comparative piRNAs Profiles Give a Clue to Transgenerational Inheritance of Sex-Biased piRNAs in Cynoglossus semilaevis

作者：Zhao, Na[1,2,3,4];Jia, Lei[5];Deng, Qiuxia[1,2];Zhu, Chunhua[1,2];Zhang, Bo[1,2]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524000, Guangdong, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab, Zhanjiang, Peoples R China;[3]Shanghai Ocean Univ, Minist Educ, Key Lab Explorat & Utilizat Aquat Genet Resources, Shanghai, Peoples R China;[4]Shanghai Ocean Univ, Int Res Ctr Marine Biosci, Minist Sci & Technol, Shanghai, Peoples R China;[5]Tianjin Fisheries Res Inst, Tianjin 300201, Peoples R China

年份：2022

卷号：24

期号：2

起止页码：335

外文期刊名：MARINE BIOTECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000769312200001)、、EI(收录号：20221111800717)、Scopus(收录号：2-s2.0-85126281124)、WOS

基金：This work was supported by grants from the Tianjin Natural Science Foundation (17JCQNJC15000), the Transformation Project of Tianjin Agricultural Achievements (201604090), the Special Funding for Modern Agricultural Industrial Technology System (CARS47-Z01), and the Funding Project of Southern Marine Science and Engineering Guangdong Laboratory-Zhanjiang 2019 (ZJW-2019-06).

语种：英文

外文关键词：Piwi-interacting RNAs; Sperm; Cynoglossus semilaevis; Male-specific biomarkers; Sex biased

外文摘要：Piwi interacting RNAs (piRNAs) are involved in the epigenetic and post-transcriptional gene silencing of retrotransposons in germ line cells, especially in spermatogenesis. There are many related reports on model organisms, such as flies and mice. In fish, however, there are few studies on piRNAs. Cynoglossus semilaevis, a benthic warm water flatfish, with remarkable sexual dimorphism, especially the "pseudo males" with sex reversal, mating with normal females to produce viable offspring, is an ideal material for the study of sex development. Here, sperm piwi-interacting RNAs profiles of Cynoglossus semilaevis were characterized, comparing between male and pseudomale groups. Differential piRNAs were identified with their predicted and annotated targets. Attention was then focused on candidate piRNAs associated with sex development and methylation. We continued to compare the expression levels of 10 candidates differentially expressed piRNAs in F1 spermatozoa. Quantitative RT-PCR demonstrated that five of the ten piRNAs showed sex bias consistent with parental sequencing results, with four significantly higher expression level in sperm of five males offspring than that of pseudomales, while one piRNAs showed the opposite expression profile. The five signature piRNAs (piR-mmu-49600337, piR-mmu-95849, piR-xtr-7474223, piR-xtr-1790334, and piR-mmu-4491546) could be employed as male-specific molecular biomarkers for C. semilaevis. Besides, this study also implied the possibility of transgenerational inheritance of sex-biased piRNAs exiting in sperm of Cynoglossus semilaevis.