文献类型：期刊文献

英文题名：Identification of a cellular role of hemolysin co-regulatory protein (Hcp) in Vibrio alginolyticus modulating substrate metabolism and biofilm formation by cAMP-CRP

作者：Wu, Shuilong[1,3,4];Huang, Yu[1,3];Wu, Minhui[4];Chen, Huapu[1,3];Wang, Bei[1,3];Amoah, Kwaku[1,3];Cai, Jia[1,2,3];Jian, Jichang[1,3]

机构：[1]Guangdong Ocean Univ, Coll Fishery, Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 518120, Peoples R China;[3]Guangdong Ocean Univ, Key Lab Dis Controlling Aquat Econ Anim, Coll Fishery, Guangdong Higher Educ Inst, Zhanjiang 524088, Peoples R China;[4]Cent Peoples Hosp Zhanjiang, Zhanjiang Inst Clin Med, Zhanjiang 524045, Peoples R China

年份：2024

卷号：282

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：SCI-EXPANDED(收录号：WOS:001342560200001)、、EI(收录号：20244317250816)、Scopus(收录号：2-s2.0-85206899194)、WOS

基金：We thank Dr. Xin Dai for the guidance in the preparation of mouse immune serum. This work was funded by the National Natural Science Foundation of China (U20A2065, 32002426, 32073006) ; Natural Sci-ence Foundation of Guangxi Province (2020GXNSFAA297243) ; Natural Science Foundation of Guangdong Province (2022A1515010553) ; Sci-ence and Technology Plan of Guangdong province (2023B0202010016) ; Youth Science and Technology Innovation Talent of Guangdong TeZhi plan talent (2023TQ07A888) ; Medical Scientific Research Foundation of Guangdong Province (A2024221) ; Science and Technology Develop-ment Special Fund Competitive Allocation Project of Zhanjiang City (2021A05117) .

语种：英文

外文关键词：Hcp; Metabolism; cAMP-CRP

外文摘要：Cyclic AMP (cAMP) and cAMP receptor protein (CRP) system controls catabolic enzyme expression based on metabolite concentrations in bacteria. Hemolysin co-regulatory protein (Hcp) is well known as a molecular chaperone for virulence factor secretion of the type VI secretion system (T6SS). However, the intracellular role of Hcp involving in bacterial physiological processes remains unknown. To clarify that, we constructed a single hcp mutant strain and analyzed their effects on the physiological processes of Vibrio alginolyticus. The omics results revealed the extensive involvement of Hcp in the catabolic metabolism in bacteria. Simultaneously, Hcp1 and Hcp2 played opposing regulatory roles on the bacterial growth, biofilm formation, and intracellular cAMP-CRP levels during cultivation in a glucose medium. Furthermore, the interacting protein screening and coimmunoprecipitation (Co-IP) assays confirmed that the glucose-specific phosphoenolpyruvate (PEP)-phosphotransferase system (PTS) enzyme IIA component (EIIAglc) was a key interacting partner with Hcp proteins as well as class I adenylyl cyclase (AC-I) in Vibrio alginolyticus. These results indicated that, to achieve cellular homeostasis, Hcp1 and Hcp2 might exert antagonistic and synergistic effects, respectively, on the interaction between EIIAglc and AC thus cooperatively regulating intracellular cAMP-CRP production.