文献类型：期刊文献

英文题名：Molecular and functional characterization of peptidoglycan-recognition protein SC2 (PGRP-SC2) from Nile tilapia (Oreochromis niloticus) involved in the immune response to Streptococcus agalactiae

作者：Gan, Zhen[1,2,3];Chen, Shannan[1,3];Hou, Jing[1,3];Huo, Huijun[1,3];Zhang, Xiaolin[1,3];Ruan, Baiye[1,3];Laghari, Zubair Ahmed[1];Li, Li[1,3];Lu, Yishan[2];Nie, Pin[1,3]

机构：[1]Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China;[2]Guangdong Ocean Univ, Coll Fishery, 40 East Jiefang Rd, Zhanjiang 524025, Guangdong, Peoples R China;[3]Univ Chinese Acad Sci, Beijing 100049, Peoples R China

年份：2016

卷号：54

起止页码：1

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000378451700001)、、Scopus(收录号：2-s2.0-84961924592)、WOS

基金：We thank all the laboratory members for their critical reviews and comments on this manuscript. We are especially grateful for the critical comments from the anonymous reviewers. This work was supported by research grants from the National Natural Science Foundation of China (No. 31320103913).

语种：英文

外文关键词：Oreochromis niloticus; Peptidoglycan-recognition protein; Streptococcus agalactiae; Amidase activity; Antimicrobial effect

外文摘要：PGRP-SC2, the member of PGRP family, plays an important role in regulation of innate immune response. In this paper, a PGRP-SC2 gene of Nile tilapia, Oreochromis niloticus (designated as On-PGRP-SC2) was cloned and its expression pattern under the infection of Streptococcus agalactiae was investigated. Sequence analysis showed main structural features required for amidase activity were detected in the deduced amino acid sequence of On-PGRP-SC2. In healthy tilapia, the On-PGRP-SC2 transcripts could be detected in all the examined tissues, with the most abundant expression in the muscle. When infected with S. agalactiae, there was a clear time-dependent expression pattern of On-PGRP-SC2 in the spleen, head kidney and brain. The assays for the amidase activity suggested that recombinant On-PGRP-SC2 protein had a Zn2+-dependent PGN-degrading activity. Moreover, our works showed that recombinant On-PGRP-SC2 protein could significantly reduce bacterial load in target organs attacked by S. agalactiae. These findings indicated that On-PGRP-SC2 may play important roles in the immune response to S. agalactiae in Nile tilapia. (C) 2016 Elsevier Ltd. All rights reserved.