文献类型：期刊文献

英文题名：Proteomic study of hypothalamus in pigs exposed to heat stress

作者：Yu, Tian-yue[1,3];Yong, Yan-hong[1];Li, Jun-yu[1];Fang, Biao[4];Hu, Can-ying[1];Wu, Lian-yun[4];Liu, Xiaoxi[1];Yu, Zhichao[1];Ma, Xingbin[1];Patil, Yadnyavalkya[2];Gooneratne, Ravi[2];Ju, Xiang-hong[1,3]

机构：[1]Guangdong Ocean Univ, Dept Vet Med, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 518018, Peoples R China;[3]Guangdong Ocean Univ, Dept Anim Sci, Zhanjiang 524088, Peoples R China;[4]Lincoln Univ, Fac Agr & Life Sci, Lincoln 7647, New Zealand

年份：2020

卷号：16

期号：1

外文期刊名：BMC VETERINARY RESEARCH

收录：SCI-EXPANDED(收录号：WOS:000562800400002)、、Scopus(收录号：2-s2.0-85089618192)、WOS

基金：This work was supported by the Project of Enhancing School with Innovation at Guangdong Ocean University (grant number 230419057), National Natural Science Foundation of China (grant nos. 31101862, 31472243) and Shenzhen Projects for Basic Research (JCYJ20170306162414058, JCYJ20190813142005766). The funding bodies had no role in the design of the research, the collection, analysis, and interpretation of data, and the writing of the manuscript.

语种：英文

外文关键词：Pigs; Heat stress; Hypothalamus; Quantitative proteomics

外文摘要：Background With evidence of warming climates, it is important to understand the effects of heat stress in farm animals in order to minimize production losses. Studying the changes in the brain proteome induced by heat stress may aid in understanding how heat stress affects brain function. The hypothalamus is a critical region in the brain that controls the pituitary gland, which is responsible for the secretion of several important hormones. In this study, we examined the hypothalamic protein profile of 10 pigs (15 +/- 1 kg body weight), with five subjected to heat stress (35 +/- 1 degrees C; relative humidity = 90%) and five acting as controls (28 +/- 3 degrees C; RH = 90%). Result The isobaric tags for relative and absolute quantification (iTRAQ) analysis of the hypothalamus identified 1710 peptides corresponding to 360 proteins, including 295 differentially expressed proteins (DEPs), 148 of which were up-regulated and 147 down-regulated, in heat-stressed animals. The Ingenuity Pathway Analysis (IPA) software predicted 30 canonical pathways, four functional groups, and four regulatory networks of interest. The DEPs were mainly concentrated in the cytoskeleton of the pig hypothalamus during heat stress. Conclusions In this study, heat stress significantly increased the body temperature and reduced daily gain of body weight in pigs. Furthermore, we identified 295 differentially expressed proteins, 147 of which were down-regulated and 148 up-regulated in hypothalamus of heat stressed pigs. The IPA showed that the DEPs identified in the study are involved in cell death and survival, cellular assembly and organization, and cellular function and maintenance, in relation to neurological disease, metabolic disease, immunological disease, inflammatory disease, and inflammatory response. We hypothesize that a malfunction of the hypothalamus may destroy the host physical and immune function, resulting in decreased growth performance and immunosuppression in heat stressed pigs.