文献类型：期刊文献

英文题名：Potential mechanisms of different methylation degrees of pectin driving intestinal microbiota and their metabolites to modulate intestinal health of Micropterus salmoides

作者：Liu, Yu;Zhou, Hang;Fan, Jiongting;Huang, Huajing;Deng, Junming[1];Tan, Beiping[1]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China; Aquat Anim Precis Nutr & High Efficiency Feed Engn, Zhanjiang 524088, Peoples R China; Minist Agr, Key Lab Aquat Livestock & Poultry Feed Sci & Techn, Zhanjiang 524088, Peoples R China

年份：2023

卷号：251

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：SCI-EXPANDED(收录号：WOS:001068410900001)、、EI(收录号：20234014818067)、Scopus(收录号：2-s2.0-85172456475)、WOS

基金：The authors thank Dr. Jiang Hui for her help with data analysis. The authors also thank the National Natural Science Foundation of China (Grant nos. 32273152 and 31760761) , Postgraduate Education Innovation Project of Guangdong Ocean University (Grant no. 202246) , National Key Research and Development Program of China (Grant no. 2019YFD0900200) , and Program for Scientific Research Startup Funds of Guangdong Ocean University (Grant no. 060302022007) for their support.

语种：英文

外文关键词：Pectin; Largemouth bass; Methylation degree; Intestinal flora; Intestinal health; Esterification degrees

外文摘要：Four diets containing 8 % cellulose, low methyl-esterified pectin (LMP), high methyl-esterified pectin (HMP) and MMP (half LMP and half HMP) were designed to evaluate the potential mechanisms by which different esterification degrees of pectin drive intestinal microbiota and their metabolites modulating the intestinal health of Micropterus salmoides. The results showed that both dietary LMP and HMP consistently upregulated intestinal zonula occludens protein 1 (Zo-1), Caludin-1, and Caludin-4, and downregulated intestinal tumor necrosis factoralpha (TNF-alpha), interleukin-8 (IL-8), and interleukin-1 beta (IL-1 ss) gene expression (P < 0.05). Dietary HMP separately upregulated intestinal Occludin, nuclear factor erythroid2-related factor 2 (Nrf2), B-cell lymphoma-2 (Bcl-2), and Bcl-2 associated agonist of cell death (BAD) gene expression, as well as the digesta propionate content, OTUs, Sobs, Shannon, Chao, and ACE indices (P < 0.05), whereas dietary LMP decreased digesta arginine, 4-aminobutyric, L-tyrosine, and phenylalanine contents (P < 0.05). Moreover, dietary HMP decreased plasma lipopolysaccharide and d-lactic acid contents and increased intestinal superoxide dismutase and glutathione peroxidase activities and immunoglobulin (Ig) receptor and IgM levels (P < 0.05). Collectively, dietary HMP improves intestinal health by increasing intestinal flora a-diversity and enhancing intestinal mechanical barrier, anti-inflammatory, antioxidant, and immune functions. On the contrary, the interference of dietary LMP with butyrate, tyrosine, arginine, and 4-aminobutyric acid metabolism is the main reason for its detrimental effects on intestinal health.