文献类型：期刊文献

英文题名：Effects of replacing microalgae with an artificial diet on pearl production traits and mineralization-related gene expression in pearl oyster Pinctada fucata martensii

作者：Yang, Chuang-ye[1];Wang, Qing-heng[1,2];Hao, Rui-juan[1,2];Liao, Yong-shan[1];Du, Xiao-dong[1,2];Deng, Yue-wen[1,2]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang, Peoples R China;[2]Pearl Breeding & Proc Engn Technol Res Ctr Guangd, Zhanjiang, Peoples R China

年份：2017

卷号：48

期号：10

起止页码：5331

外文期刊名：AQUACULTURE RESEARCH

收录：SCI-EXPANDED(收录号：WOS:000409471300015)、、Scopus(收录号：2-s2.0-85018602095)、WOS

基金：Department of Science and Technology of Guangdong Province, Grant/Award Number: 2014A020208122; China Agriculture Research System, Grant/Award Number: CARS-048; Guangdong Marine and Fishery Bureau, Grant/Award Number: Z2014009 and A201308A06

语种：英文

外文关键词：artificial diet; growth gene; mineralization gene; pearl production traits; Pinctada fucata martensii

外文摘要：The effects of replacing dietary microalgae with artificial diet on pearl production traits and biomineralization-related gene expression were investigated in pearl oyster (Pinctada fucata martensii). Three experiment groups (EG1, EG2 and EG3) were set. EG1 and EG3 were separately fed Platymonas subcordiformis and artificial diet (D4), respectively, and EG2 was fed with mixed P.subcordiformis and D4. A control group (CG) was cultured in natural sea. All groups were continuously fed for 150days. The results showed that the survival rates of EG1, EG2 and EG3 were significantly higher than that of CG. No significant differences in retention rate and pearl thickness were observed among the four groups, but CG had the lowest values of both parameters. The relative expression levels of EGFR and FGF18 mRNA did not significantly differ among EG1, EG2 and EG3. Moreover, the relative expression levels of GHITM and TR I mRNA significantly varied among the groups and EG3, and EG1 had the highest and lowest relative expression levels of GHITM and TR I mRNA respectively. The relative expression levels of nacrein and chitin-binding mRNA significantly differed among the groups, and EG1 had the lowest expression levels of nacrein and chitin-binding mRNA. No significant differences in the relative expression levels of MSI60 and TIMP mRNA were observed among the groups. The results suggested that D4 can replace part of microalgae, which is helpful for further studies on developing artificial diet of pearl oyster.