文献类型：期刊文献

英文题名：Dietary supplementation with polypeptides improved growth performance, antibacterial immune and intestinal microbiota structure of Litopenaeus vannamei

作者：Liao, Xu-zheng[1];Hu, Shi-kang[1];Wang, Bo[1];Qin, Hai-peng[1];Zhao, Ji-chen[1];He, Zi-hao[1];Chen, Xie-yan[1];Liu, Yong-sheng[2];Qu, Peng[3];Sun, Cheng-bo[1];Zhang, Shuang[1]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China;[2]Yantai Aquat Res Inst, Yantai, Peoples R China;[3]Shangdong Hypobenthos Inc Co, Yantai, Peoples R China

年份：2019

卷号：92

起止页码：480

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000483634300054)、、WOS

基金：This study was supported by National Natural Science Foundation of China (NSFC 31702377); Free application Fund of Natural Science Foundation of Guangdong Province, China (2016A030313757); Special Scientific and Technological Research and Development Project for Marine Fisheries Science and Technology Development of Guangdong Province(A201508805) and Yantai City's "Thirteen Five-Year National Marine Economic Innovation and Development Demonstration City" marine bio-innovation sector and industrial chain demonstration construction project (2017yy09).

语种：英文

外文关键词：Polypeptide; Litopenaeus vannamei; Growth performance; Antibacterial immune; Intestinal microbiota

外文摘要：Antibacterial peptides (AMPS) are expected to replace some or all of the antibiotics and become a new feed additive. However, the high production cost and unclear mechanism limited the application of AMPS. In this research, the effects of a commercial polypeptide (Polypeptide 5100) whose main components are AMPS on the growth, antibacterial immune and intestinal microbial of Litopenaeus vannamei were study. L. vannamei (initial weight of 0.16 +/- 0.03 g) were fed for 123 days with basal diet added Polypeptide 5100 at two levels each (0.5% and 1%) as experimental groups, and a basal diet as control. Dietary inclusion of Polypeptide 5100 at 1% level significantly increased the weight gain (WG) and specific growth rate (SGR) of L. vannamei. The survival rates of L. vannamei in 0.5% and 1% Polypeptide S100 groups were significantly higher than the control when infected by Vibrio harveyi but not Vibrio parahaemolyticus. The activities of total superoxide dismutase (T-SOD) and lysozyme (LZM) in the two experimental groups were all significantly higher than the control. Differently, the activities of amylase (AMS) and lipase (LPS) were significantly higher in 0.5% Polypeptide 5100 group but lower in 1.0% Polypeptide S100 group. Illumina MiSeq high-throughput sequencing showed that the dominant phyla in the intestine of L. vannamei were Proteobacteria, followed by Actinobacteria, Bacteroidetes, Chloroflexi, Cyanobacteria, Fusobacteria and Tenericutes, and the abundance of predominant phyla Cyanobacteria were upregulated significantly in the experimental groups. At the family level, significant increase was observed in Pseudomonadaceae and Xanthomonadaceae but decrease in Vibrionaceae in the 1.0% Polypeptide 5100 group. The abundance of predominant genus Photobacteterium were obviously downregulated in the two experimental groups. Unlikely, the abundance of Pseudomonas and Stenotrophomonas were distinctly increased in the 1.0% Polypeptide S100 group but not significantly different from the control in 0.5% Polypeptide 5100 group. All these results suggested that Polypeptide S100 could improve the growth performance, antibacterial immune and intestinal microbiota structure of L. vannamei.