文献类型：期刊文献

中文题名：复合益生菌对杂交石斑鱼生长性能、抗氧化能力和肠道健康的影响

英文题名：Effects of Compound Probiotics on Growth Performance,Antioxidant Capacity and Intestinal Health of Hybrid Grouper(Epinephelus fuscogutatus♀×Epinephelus polyphekadion♂)

作者：蒋鑫涛[1];陈有铭[2];黄鉴鹏[1];欧光海[1];温震威[1];李豫[1];马骞[1];陈刚[1,3]

机构：[1]广东海洋大学水产学院,广东湛江524088;[2]广东蓝粮种业有限公司,广东湛江524000;[3]广东省水产动物病害防控与健康养殖重点实验室,广东湛江524088

年份：2023

卷号：43

期号：5

起止页码：81

中文期刊名：广东海洋大学学报

外文期刊名：Journal of Guangdong Ocean University

收录：CSTPCD、、CSCD_E2023_2024、北大核心、CSCD、北大核心2020

基金：国家重点研发计划项目(2022YFD2401203)。

语种：中文

中文关键词：杂交石斑鱼;复合益生菌;生长性能;抗氧化;肠道组织形态;肠道菌群

外文关键词：Epinephelus fuscogutatus♀×Epinephelus polyphekadion♂;compound probiotics;growth performance;antioxidant;intestinal tissue morphology;intestinal microflora

中文摘要：【目的】探究饲料中添加由枯草芽孢杆菌(Bacillus subtilis)和嗜酸乳杆菌(Lactobacillus acidophilus)组成的复合益生菌对杂交石斑鱼(Epinephelus fuscogutatus♀×Epinephelus polyphekadion♂)生长性能、抗氧化能力、肠道组织形态和肠道菌群的影响。【方法】将复合益生菌粉按照0%、0.01%、0.10%和1.00%的质量比例分别添加到杂交石斑鱼的基础饲料中,投喂22 d后测定其生长指标、肠道抗氧化酶活性,HE染色切片观察肠道组织形态与结构,高通量测序分析肠道菌群结构。【结果】与对照组相比,添加组的存活率、特定生长率、增重率无显著差异(P>0.05);添加组MDA活性均显著降低(P<0.05),0.10%组的SOD活性显著升高(P<0.05);添加组的后肠肌层厚度显著增加(P<0.05);后肠绒毛宽度与黏膜层上皮内杯状细胞数量均随着复合益生菌添加量的增加而增加;菌群丰富度(ACE和Chao1)有所增加,但没有显著差异(P>0.05);菌群多样性(Simpson和Shannon)显著增加(P<0.05);添加组鱼肠道菌群中严格梭菌属(Clostridium sensu stricto 1)和Turicibacter的种类相对丰度显著减少(P<0.05);乳杆菌目(Lactobacillales)、拟杆菌门(Bacteroidota)、放线菌门(Actinobacteriota)、颤螺菌目(Oscillospirales)、瘤胃球菌科(Ruminococcaceae)和Muribaculaceae中的种类相对丰度显著增加(P<0.05);功能预测分析中,添加组鱼的碳代谢、不同环境下的微生物代谢、抗生素的生物合成和次级代谢产物的生物合成相比对照组普遍显著上调(P<0.05)。【结论】饲料中添加该种复合益生菌可改善杂交石斑鱼肠道组织形态及消化吸收功能,强化肠道的抗氧化能力,提高益生菌的相对丰度,加强了肠道菌群对碳源的有效利用,改善肠道免疫平衡,对杂交石斑鱼的肠道健康具有积极作用。

外文摘要：【Objective】To investigate the effects of compound probiotics composed of Bacillus subtilis and Lactobacillus acidophilus on growth performance,antioxidant capacity,intestinal morphology and intestinal microflora of hybrid grouper(Epinephelus fuscogutatus♀×Epinephelus polyphekadion♂).【Method】Compound probiotics powder was added to the basal diet of hybrid grouper at the mass ratio of 0%,0.01%,0.10%and 1.00%respectively.After feeding for 22 days,the growth indexes,intestinal antioxidant enzyme activities were measured;the intestinal tissue morphology and structure were observed by HE staining sections;and the intestinal microflora structure was analyzed by high throughput sequencing.【Result】Compared with control(0%)group,SR(survival rate),SGR(specific growth rate)and WGR(weight gain rate)in experiment(0.01%,0.10%and 1.00%)groups had no significant differences(P>0.05);MDA activity was significantly decreased(P<0.05),and SOD activity in 0.10%group was significantly increased(P<0.05);the thickness of posterior intestinal muscle layer in experiment groups was significantly increased(P<0.05);the width of villi in the posterior intestine and the number of goblet cells in mucosal layer increased with the increase of the proportion of complex probiotics;microflora richness(ACE and Chao1)increased,but there was no significant difference(P>0.05);microflora diversity(Simpson and Shannon)was significantly increased(P<0.05).The relative abundency of Clostridium sensu stricto 1 and Turicibacter in the intestinal microflora of theexperiment groups decreased significantly(P<0.05),the relative abundency of Lactobacillales,Bacteroidota,Actinobacteriota,Oscillospirales,Ruminococcaceae and Muribaculaceae increased significantly(P<0.05).In the functional prediction analysis,the carbon metabolism,microbial metabolism,antibiotic biosynthesis and secondary metabolites biosynthesis in the experiment groups were generally significantly up-regulated compared with the control group(P<0.05).【Conclusion】Dietary supplementation of this compound probiotics can improve intestinal tissue morphology and digestive and absorption function of hybrid grouper,strengthen intestinal antioxidant capacity,improve the relative abundance of probiotics,enhance intestinal microflora's effective utilization of carbon sources,improve intestinal immune balance,and reduce the induction of intestinal inflammation.It has positive effect on intestinal health of hybrid grouper.