文献类型：期刊文献

英文题名：A novel aflatoxin B-1 biosensor based on a porous anodized alumina membrane modified with graphene oxide and an aflatoxin B-1 aptamer

作者：Mo, Rijian[1];He, Lei[2];Yan, Xiemin[1];Su, Tiantian[1];Zhou, Chunxia[1,3];Wang, Zhe[4];Hong, Pengzhi[1,3];Sun, Shengli[2];Li, Chengyong[2,3]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Sch Chem & Environm, Zhanjiang 524088, Peoples R China;[3]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 518108, Peoples R China;[4]Chinese Acad Sci, Shenzhen Inst Adv Technol, Ctr Biomed Mat & Interfaces, Shenzhen 518055, Peoples R China

年份：2018

卷号：95

起止页码：9

外文期刊名：ELECTROCHEMISTRY COMMUNICATIONS

收录：SCI-EXPANDED(收录号：WOS:000446243500003)、、EI(收录号：20183605765694)、Scopus(收录号：2-s2.0-85052466167)、WOS

基金：This work was supported by the National Natural Science Foundation of China (21874029), Science and Technology Planning Project of Guangdong Province (2016A020210114), Science and Technology Planning Project of Shenzhen City (JCYJ20170818111719650), Industrial Development Special Funds of Dapeng New Area (KY20170209), Natural Science Foundation of Guangdong Ocean University (C17381), Innovation and Development Project about Marine Economy Demonstration of Zhanjiang City (2017C8B1) and Graduate Education Innovation Program of Guangdong Ocean University (201733).

语种：英文

外文关键词：Biosensor; Nanochannels; Aptamer; Graphene oxide; Porous alumina membrane; Aflatoxin B-1

外文摘要：A novel aflatoxin B-1 biosensor is fabricated from a porous anodized alumina membrane modified with graphene oxide and an aptamer of aflatoxin B-1. Briefly, the aptamer is immobilized on the surface of the porous anodized alumina nanochannels by covalent bonding. Graphene oxide is then added to the surface by pi-pi stacking with the aptamer. The negative charge of the nanochannels is increased by the introduction of the negatively charged graphene oxide and the aptamer, which also cause steric hindrance. On the addition of aflatoxin B-1, graphene oxide is detached from the surface of the nanochannels due to the specific binding of aflatoxin B-1 to the aptamer. The flux of Fe(CN)(6)(3-) through the nanochannels is increased because the charge density and steric hindrance are reduced, resulting in an increased current response. The increase in current is proportional to the concentration of aflatoxin B-1. The detection limit of the proposed biosensor is about 0.13 ng/mL and the linear range is 1-20 ng/mL. Furthermore, this sensor has excellent selectivity towards aflatoxin B-1, offering the potential for a sensitive, simple and effective method for detecting mycotoxins.