文献类型：期刊文献

英文题名：Effect of T-2 toxin-injected shrimp muscle extracts on mouse macrophage cells (RAW264.7)

作者：Huang, Zhanrui[1];Wang, Yaling[1];Qiu, Mei[1];Sun, Lijun[1];Liao, Jianmeng[1];Wang, Rundong[1];Sun, Xiaodong[2];Bi, Siyuan[3];Gooneratne, Ravi[4]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Zhanjiang, Peoples R China;[2]Dalian Nationalities Univ, Coll Environm & Resources, Dalian, Peoples R China;[3]Shenzhen Bioeasy Biotechnol Co Ltd, Shenzhen, Peoples R China;[4]Lincoln Univ, Dept Wine Food & Mol Biosci, Ctr Food Res & Innovat, Canterbury, New Zealand

年份：2018

卷号：41

期号：1

起止页码：16

外文期刊名：DRUG AND CHEMICAL TOXICOLOGY

收录：SCI-EXPANDED(收录号：WOS:000417762600003)、、Scopus(收录号：2-s2.0-85012053887)、WOS

基金：This study was financially supported by the National Natural Science Foundation of China (NSFC) (Nos. 31171634, 31371777, and 31200014).

语种：英文

外文关键词：Shrimp; T-2 toxin; accumulation; in-vitro digestion; immunocytotoxicity

外文摘要：Following intramuscular injections of 0.1 mL, 3 mg kg(-1) BW-1(1/10 LD50) T-2 toxin (T-2), the tissue concentration of T-2 in shrimp was quantitatively detected using LC-MS/MS. The biological half-time (t(1/2)) of T-2 in blood was 40.47 +/- 0.24 min. The highest number of intramuscular T-2 shrimp could tolerate when given at blood t(1/2) intervals was 4. The shrimps which were injected 5 T-2 died. The T-2 toxin highest accumulation was 0.471 +/- 0.012 ng g(-1) BW-1. The effect of toxic shrimp muscle subjected to different processing conditions (high pressure, trifluoroacetic acid, acid and alkali digestions, artificial digestive juice [to simulate exposure to gastric and intestinal juices]) on mouse macrophage cells (RAW267.4) were evaluated by the MTT assay. The inhibition ratio of 2% muscle extract on RAW267.4 was 85.70 +/- 2.63%. The immunocytotoxicity of muscle extracts to RAW264.7 was highest in muscle extracts subjected to physical and chemical digestion (high pressure > NaOH > trifluoroacetic acid > 0.02 M HCl > 0.2 M HCl > controls), and also artificial digestion (artificial intestinal juice > artificial gastric juice > N type intestinal juice > N type gastric liquid > controls). Results showed that high-pressure and artificial intestinal juice were most effective in the release of modified T-2 to free T-2 thus enhancing toxicity. These results can be interpreted as measurement of T-2 in food being of little value because of enhanced toxicity of T-2-contaminated food as they pass through the gastrointestinal tract.