文献类型：期刊文献

英文题名：Molecular cloning and dimorphic expression of growth hormone (gh) in female and male spotted scat Scatophagus argus

作者：Deng, Si-Ping[1,2];Wu, Bo[1,2];Zhu, Chun-Hua[1,2];Li, Guang-[1,2]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Guangdong Higher Educ Inst, Key Lab Aquaculture Souh China Sea Aquat Econ Ani, Zhanjiang 524088, Peoples R China

年份：2014

卷号：80

期号：4

起止页码：715

外文期刊名：FISHERIES SCIENCE

收录：SCI-EXPANDED(收录号：WOS:000339334200008)、、WOS

基金：This work was supported by grants from the National Natural Science Foundation of China (No. 31272640) and the Science and Technology Projects of Zhanjiang (No. 2012C3102001).

语种：英文

外文关键词：Growth hormone; Pituitary; Scatophagus argus; Sexual growth dimorphism

外文摘要：Spotted scat Scatophagus argus exhibits a typical sexual growth dimorphism in which the females grow faster than the males. Growth hormone (GH) is best known as an anterior pituitary hormone fundamental in regulating growth. To clarify the roles in sexual growth dimorphism in the spotted scat, gh cDNA was isolated from the pituitary. A phylogenetic tree was constructed based on the GH amino acid sequences of spotted scat and other vertebrates, and the resulting topology clearly reflects the taxonomic relationship of the Perciformes species selected. Alignment of GH amino acid sequences displayed very high similarity between the spotted scat and the other Perciformes species. The qRT-PCR analysis demonstrated that females exhibited higher pituitary gh mRNA levels than males at 6, 18, and 30 months (P < 0.05), which was 1.84, 4.61 and 6.34 times greater, respectively. In addition, immature males (6 months) exhibited higher pituitary gh mRNA levels than mature males (18 and 30 months). These results imply that the sexual growth dimorphism may be ascribable to the gh levels in pituitary in spotted scat.