文献类型：期刊文献

英文题名：Triplex DNA-based aggregation-induced emission probe: A new platform for hybridization chain reaction-based fluorescence sensing assay

作者：Li, Yubin[1,2];Li, Ciling[1];Zhang, Chuangqiang[1];Zhao, Liting[1];Huang, Yaoyun[1]

机构：[1]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Res Ctr Coastal Environm Protect & Ecol Resilience, Zhanjiang 524088, Peoples R China

年份：2024

卷号：1299

外文期刊名：ANALYTICA CHIMICA ACTA

收录：SCI-EXPANDED(收录号：WOS:001209075700001)、、EI(收录号：20241015680131)、Scopus(收录号：2-s2.0-85186384309)、WOS

基金：This work was supported by the project of Guangdong Natural Science Foundation, China (2023A1515011157) , Guangdong Province Science and Technology Innovation Strategy Special Fund, China (pdjh2023b0251) , National college students innovation and entrepreneurship training program, China (202310566003X) and the Guangdong Ocean University Innovation and Entrepreneurship Team Project (CCTD201830) .

语种：英文

外文关键词：Aggregation-induced emission; Fluorescence probe; Triplex DNA; Hybridization chain reaction

外文摘要：The hybridization chain reaction (HCR), as one of the nucleic acid amplification technologies, is combined with fluorescence signal output with excellent sensitivity, simplicity, and stability. However, current HCR-based fluorescence sensing methods still have some defects such as the blocking effect of the HCR combination with fluorophores and the aggregation -caused quenching (ACQ) phenomenon of traditional fluorophores. Herein, a triplex DNA -based aggregation -induced emission probe (AIE-P) was designed as the fluorescent signal transduction, which is able to provide a new platform for HCR-based sensing assay. The AIE-P was synthesized by attaching the AIE fluorophores to terminus of the oligonucleotide through amido bond, and captured the products of HCR to form triplex DNA. In this case, the AIE fluorophores were located in close proximity to generate fluorescence. This assay provided turn -on fluorescence efficiency with a high signal-to-noise ratio and excellent amplification capability to solve the shortcoming of HCR-based fluorescence sensing methods. It enabled sensitive detection of Vibrio parahaemolyticus in the range of 102-106 CFU mL- 1, and with a low limit of detection down to 39 CFU mL-1. In addition, this assay expressed good specificity and practicability. The triplex DNA -based AIE probe forms a universal molecular tool for developing HCR-based fluorescence sensing methods.