文献类型：期刊文献

英文题名：Progesterone and 17β-estradiol, but not follicle stimulating hormone, alter the sex ratio of murine embryos fertilized in vitro

作者：Zhang, L.[1,2];Du, W.[1];Lin, X.[1];Zhang, A.[3];Chen, H.[3]

机构：[1]Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100094, Peoples R China;[2]Guangdong Ocean Univ, Coll Agr, Zhanjiang 524025, Peoples R China;[3]NW A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Peoples R China

年份：2008

卷号：69

期号：8

起止页码：961

外文期刊名：THERIOGENOLOGY

收录：SCI-EXPANDED(收录号：WOS:000255164600008)、、WOS

语种：英文

外文关键词：sex ratio; IVF; blastocyst; diplex nested PCR; balb/c mouse

外文摘要：The objective was to determine the effects of adding progesterone, 17 beta-estradiol (17 beta-E-2), and FSH during in vitro fertilization on development and sex ratio of murine embryos. Progesterone (33 - 330 pg/mL), 17 beta-estradiol (17 beta-E-2, 10 - 70 pg/mL), and FSH (0.01 - 0.05 IU/mL), were added to human tubal fluid (HTF); this medium (with or without hormones) was used to pre-incubate sperm (2 h) and to co-incubate sperm and oocytes (6 h). Thereafter, the ova were washed and incubated in mM16 medium and embryo sex was determined (by PCR) on Day 4 (insemination = Day 0). There was no effect (P > 0.05) of hormone treatments on rates of cleavage (6 h after cessation of co-incubation with sperm). The only significant effects of added hormones on development were a decrease in the rate of development to at least the morula stage in 165 pg/mL progesterone (0.46 +/- 0.03 vs. 0.54 +/- 0.05 in the control, mean +/- S.D.; P < 0.05) and a decrease in the blastocyst rate in 0.03 IU/mL FSH (0.34 +/- 0.00 vs. 0.42 +/- 0.04 in the control, P < 0.05). However, the ratio of male to female embryos was 1.61 and 2.90 following the addition of 99 pg/mL progesterone and 70 pg/mL 17 beta-E-2, respectively; both of these ratios were different (P < 0.01) than in the control group (1.20). In contrast, the addition of FSH to the medium had no significant effect on this ratio (range, 0.78 - 1.02). We concluded that the addition of progesterone and estradiol to the media during in vitro fertilization did not enhance embryonic development, but significantly increased the proportion of male murine embryos. (c) 2008 Elsevier Inc. All rights reserved.