文献类型：期刊文献

英文题名：Comparison of Gonadal Transcriptomes Uncovers Reproduction-Related Genes with Sexually Dimorphic Expression Patterns in Diodon hystrix

作者：Chen, Huapu[1];Li, Zhiyuan[1];Wang, Yaorong[1];Huang, Hai[2];Yang, Xuewei[3];Li, Shuangfei[3];Yang, Wei[4];Li, Guangli[1]

机构：[1]Guangdong Ocean Univ, Key Lab Marine Ecol & Aquaculture Environm Zhanji, Coll Fisheries, Guangdong Res Ctr Reprod Control & Breeding Techn, Zhanjiang 524088, Peoples R China;[2]Hainan Trop Ocean Univ, Key Lab Utilizat & Conservat Trop Marine Bioresou, Minist Educ, Sanya 572022, Peoples R China;[3]Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen Key Lab Marine Bioresource & Ecoenvironm, Shenzhen 518060, Peoples R China;[4]Yangjiang Polytech, Food & Environm Engn Dept, Yangjiang 529566, Peoples R China

年份：2021

卷号：11

期号：4

外文期刊名：ANIMALS

收录：SCI-EXPANDED(收录号：WOS:000642687500001)、、Scopus(收录号：2-s2.0-85103636212)、WOS

基金：This research was funded by grants from the Key R&D Project of Hainan Province (grant number ZDYF2018225), Major Science and Technology Plan Projects of Hainan Province (grant number ZDKJ2016009), Natural Science Foundation of Guangdong Province (grant number 2019A1515010958), College of Hainan Tropical Marine Project Leader and Doctoral Research Grant 2016 (grant number RHDXB201612), The Open Project of Key Laboratory of Utilization and Protection of Tropical Marine Biological Resources (Hainan Tropical Ocean University), Ministry of Education (grant number UCTMB20201), Young Creative Talents Project of Guangdong Province Universities and Colleges (grant number 2017GkQNCX092), Guangdong Basic and Applied Basic Research Foundation (grant number 2019A1515110290), and China Postdoctoral Science Foundation (grant number 2020M682832).

语种：英文

外文关键词：Diodon hystrix; transcriptome; gonad; reproduction; sex-biased genes

外文摘要：Simple Summary Spot-fin porcupine fish (Diodon hystrix) has been recognized as a new and emerging aquaculture species with promising economic value in south China. However, due to the lack of understanding of reproductive regulation, the management of breeding and reproduction under captivity remains a major technological barrier for the development of large-scale aquaculture of D. hystrix. In this study, the first gonad transcriptomes of D. hystrix were analyzed using Illumina HiSeq sequencing. Comparison of ovary and testis transcriptomes identified a set of differentially expressed genes (DEGs) supposed to be associated with gonadal development and gametogenesis. The conserved expression profiles of the well-known reproduction-related genes implies their similar roles in gonad differentiation and development in D. hystrix. The detailed transcriptome data can improve our understanding of the regulatory functions of sex-related genes in D. hystrix. Diodon hystrix is a new and emerging aquaculture species in south China. However, due to the lack of understanding of reproductive regulation, the management of breeding and reproduction under captivity remains a barrier for the commercial aquaculture of D. hystrix. More genetic information is needed to identify genes critical for gonadal development. Here, the first gonadal transcriptomes of D. hystrix were analyzed and 151.89 million clean reads were generated. All reads were assembled into 57,077 unigenes, and 24,574 could be annotated. By comparing the gonad transcriptomes, 11,487 differentially expressed genes were obtained, of which 4599 were upregulated and 6888 were downregulated in the ovaries. Using enrichment analyses, many functional pathways were found to be associated with reproduction regulation. A set of sex-biased genes putatively involved in gonad development and gametogenesis were identified and their sexually dimorphic expression patterns were characterized. The detailed transcriptomic data provide a useful resource for further research on D. hystrix reproductive manipulation.