文献类型：期刊文献

中文题名：细毛石花菜多糖自由基清除能力和理化性质的研究

英文题名：Research on the free radical scavenging ability and physicochemical properties of the polysaccharides in Gelidium crinale

作者：裴育[1];杨胜涛[1];冯瑞[1];周春霞[1,2];洪鹏志[1,2]

机构：[1]广东海洋大学食品科技学院,广东省水产品加工与安全重点实验室,广东省海洋食品工程技术研究中心,广东湛江524088;[2]南方海洋科学与工程广东省实验室(湛江),广东湛江524025

年份：2022

卷号：48

期号：4

起止页码：235

中文期刊名：食品与发酵工业

外文期刊名：Food and Fermentation Industries

收录：CSTPCD、、CSCD2021_2022、北大核心、CSCD、北大核心2020

基金：湛江市海洋经济创新发展示范市建设项目(XM-202008-01B1)。

语种：中文

中文关键词：细毛石花菜;多糖;理化性质;抗氧化

外文关键词：Gelidium crinale;polysaccharide;physicochemical properties;anti-oxidation

中文摘要：该文通过研究细毛石花菜(Gelidium crinale)多糖提取物对自由基的清除能力及理化性质,为细毛石花菜的高值化加工利用提供实验依据。以细毛石花菜为原料,用0.1 mol/L的HCl提取制备细毛石花菜多糖,并对其理化性质和抗氧化活性进行研究。结果显示,细毛石花菜多糖中总糖占比为70.51%,还原糖占比为8.42%,硫酸基团占比为16.50%,蛋白质占比为5.27%。单糖组成分析表明,半乳糖含量最高,岩藻糖和木糖次之,相对分子质量为25.8 kDa。体外抗氧化实验结果显示,细毛石花菜多糖具有较好的抗氧化活性,其ABTS阳离子自由基清除能力、Fe^(2+)螯合能力、DPPH自由基清除能力和羟自由基清除能力的IC50分别为2.215、2.688、7.408、13.56 mg/mL。细胞实验结果显示,细毛石花莱多糖处理后细胞中的超氧化物歧化酶水平以剂量依赖的方式显著增加,对活性氧的产生有抑制作用(P<0.001)。

外文摘要：Gelidium crinaleis a marine economic red algae belonging to the Gelidium.By studying the scavenging ability and physical and chemical properties of its polysaccharides extracts on free radicals,it provides an experimental basis for the high-value processing and utilization of Gelidium crinale.In this study,Gelidium crinale was used as raw material and polysaccharides were extracted from Gelidium crinale with 0.1 mol/L HCl.Its physical and chemical properties and antioxidant activity were studied.The results showed that the proportion of total sugars and reducing sugar in polysaccharides were 70.51%and 8.42%,respectively.Moreover,the proportion of sulfate group and protein were 16.50%and 5.27%.Analysis of monosaccharide composition showed that the top three were galactose,fucose and xylose which with a relative molecular weight of 25.8 kDa.The results of in vitro antioxidant results showed that polysaccharides from Gelidium crinale had good antioxidant activity.The IC50 of its ABTS free radical scavenging ability,Fe^(2+) chelating ability,DPPH free radical scavenging ability and hydroxyl free radical scavenging ability were 2.215,2.688,7.408,13.56 mg/mL,respectively.The results of cell experiments showed that the level of superoxide dismutase(SOD)in cells was significantly increased in a dose-dependent manner after treatment with polysaccharide from Gelidium crinale,but it inhibited the production of reactive oxygen species(ROS)(P<0.001).