文献类型：期刊文献

英文题名：Fish Protein Isolates Recovered from Silver Carp (Hypophthalmichthys molitrix) By-Products Using Alkaline pH Solubilization and Precipitation

作者：Zhong, Saiyi[1];Liu, Shouchun[2];Cao, Jinxuan[3];Chen, Suhua[1];Wang, Weimin[1];Qin, Xiaoming[1]

机构：[1]Guangdong Ocean Univ, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Coll Food Sci & Technol, Zhanjiang 524025, Guangdong, Peoples R China;[2]Natl Engn Res Ctr Informat Technol Agr, Beijing, Peoples R China;[3]Ningbo Univ, Dept Food Sci & Engn, Ningbo, Zhejiang, Peoples R China

年份：2016

卷号：25

期号：3

起止页码：400

外文期刊名：JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000378703000012)、、EI(收录号：20162002398336)、Scopus(收录号：2-s2.0-84966716381)、WOS

基金：This research project is supported by the grants from Science and Technology Department of Guangdong Province (2015 B02025003), the Natural Science Foundation of Guangdong Province (No. S2012040006790), and the start-up fund from GDOU (E12319).

语种：英文

外文关键词：Silver carp processing by-products; protein recovery; acid-aided process; alkali-aided process; quality characterization

外文摘要：Fish protein isolates (FPI) were produced from silver carp processing by-products by means of the isoelectric solubilization-precipitation method, and the quality was evaluated. The alkali-aided process led to significantly more lipid reduction (p < 0.05) compared with the acid-aided process. Similar protein types were extracted for the acid and alkali solubilization, and most protein types can be recovered from the raw materials, as observed in the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The FPI was higher in total amino acids and essential amino acids (EAAs) compared with the original by-products (p < 0.05). The alkali-aided process yielded a higher nutritional and color quality than the acidic process. Furthermore, we found that alkali-aided protein had higher oxidative stability than the acid-aided protein. Our study indicates that high-quality fish proteins can be efficiently recovered from low-value silver carp processing by-products using isoelectric solubilization/precipitation, particularly the alkali-aided process, and subsequently be used in value-added human foods.