文献类型：期刊文献

英文题名：Single Nucleotide Polymorphism (SNP) Analysis of orexin Gene 5′ Regulatory Region in Chinese Indigenous Cattle Populations

作者：Zhang Ai-ling[1,2];Zhang Li[1,3];Zhang Liang-zhi[1];Lan Xian-yong[1];Zhang Cun-lei[4];Zhang Cun-fang[1];Chen Hong[1,4]

机构：[1]NW A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Peoples R China;[2]S China Agr Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou 510642, Guangdong, Peoples R China;[3]Guangdong Ocean Univ, Coll Agr, Zhanjiang 524000, Peoples R China;[4]Xuzhou Normal Univ, Coll Life Sci, Xuzhou 221000, Peoples R China

年份：2011

卷号：10

期号：8

起止页码：1273

外文期刊名：AGRICULTURAL SCIENCES IN CHINA

收录：SCI-EXPANDED(收录号：WOS:000293815600016)、、WOS

基金：This study was supported by the National Natural Science Foundation of China (30972080), the National Key Technology R&D Program of China (2008BADB2B03-19), the Keystone Project of Transfergene in China (2009ZX08009-157B, 2008ZX08007-002, 2009ZX080 07-005B-07), the Program of National Beef Cattle Industrial Technology System, China (CARS-38), China Postdoctoral Science Foundation Founded Project (20100480763), and the Doctor Seed Grant of Guangdong Ocean University, China (0712130).

语种：英文

外文关键词：orexin gene; promoter; SNP; cattle

外文摘要：Orexin is an important neuropeptide that influences livestock's appetite and food intake and is closely related with livestock's growth and development. The variations in the orexin gene 5' regulatory region might have an influence on the gene expression. Based on the hypothesis, five overlapped fragments including I 794 bp of orexin gene 5' regulatory region were investigated for single nucleotide polymorphisms (SNPs) by PCR-SSCP and DNA sequencing in three indigenous cattle populations. A total of five SSCP patterns observed revealed ten SNPs in the region. Two SSCP patterns genotypes (A and B) were exhibited in O-2 fragment and three (A, B and C) were found in O-5 fragment. O-2 contained four SNPs, viz., -583 T>C, -479 C>T, -474 A>T, and -451 A>G. In another bus O-5, six SNPs were identified (-1 610 C>G, -1 585 G>A, -1 550 T>C, -1 548 A>C, -1 438 C>T, and -1431 C>A). Seven SNPs were found in transcription factor binding sites and four out of them existed in the core sequences. The SNPs at -479, -474 and -451 did not change the putative recognition core sequences of their factors. But the mutation at -583 changed the binding sequence of EVI1 into NFA and created one new binding site for ZFHX simultaneously. In three populations, the frequencies of A, B and C genotypes of O-2 were 0.2367, 0.4842 and 0.2791, respectively. And the A pattern of O-5 was preponderant (0.7549) and the other B pattern was not (0.2451). But the frequencies of different SSCP variants varied across three cattle populations. B genotype in O-2 had significant associations to body weight (BW) and daily weight gain (DWG) in Nanyang cattle 6- and 12-mon aged and might serve as one potential candidate genetic marker for growth and development.