文献类型：期刊文献

中文题名：全缘马尾藻褐藻多酚的抗氧化和抗肿瘤细胞增殖作用研究

英文题名：Antioxidant Activity and Antiproliferation Effect on Tumor Cells of Phlorotannins from Sargassum integerrimum

作者：卢虹玉[1];刘义[2];吉宏武[1];邵海艳[1]

机构：[1]广东海洋大学水产品深加工广东普通高校重点实验室,广东湛江524088;[2]广东医学院广东天然药物研究与开发实验室,广东湛江524023

年份：2013

卷号：29

期号：4

起止页码：702

中文期刊名：现代食品科技

外文期刊名：Modern Food Science and Technology

收录：CSTPCD、、北大核心2011、EI(收录号：20132116359947)、Scopus(收录号：2-s2.0-84877866073)、北大核心

基金：湛江市科技攻关计划项目(2011C31070209)

语种：中文

中文关键词：全缘马尾藻;褐藻多酚;总抗氧化能力;抗增殖

外文关键词：Sargassum integerrimum; phlorotannins; total antioxidant capacity; antiproliferation

中文摘要：检测全缘马尾藻褐藻多酚提取物（IPTs）的抗氧化和抗肿瘤细胞增殖作用。通过萃取和超滤获得4种IPTs提取物-IPTa1、IPTa2、IPTe1和IPTe2，总酚含量分别为1．16、0．6、0．25和0．34mg没食子酸（GA）／g海藻干基，1．5mg／L时的总抗氧化能力（TCA）分别相当于1．724、1．379、1．833、0．695mg／L的Fe2＋还原能力；MTT结果显示，4．4μg／mL时，4种IPTs提取物作用sw579细胞24h、48h、72h后，除了IFTa2外，IPTa1、IPTe1和IPTe2均以时间依赖方式显著抑制肿瘤细胞的生长；0．3125--5μg／mL浓度范围内，IPTa1和Igre1的抑制率与作用时间和浓度呈正相关，最高抑制率达90％以上。

外文摘要：To investigate antioxidant activity and antiproliferation effect of phlorotannins extraction from Sargassum integerrimum OPTs） IPTs was prepared by solvent extraction and ullrafiltration. Four extracts named IPTal, IgTa2, IPTel and IPTe2 were attained. Total phenolic contents of the four IPTs were 1.16, 0.6, 0.25 and 0.34 mg GA/g algae dry base. Total antioxidant capacities of the four extracts at 1.5 mg/L were equal to reduction capacities of Fe2＋at 1.724, 1.379, 1.833 and 0.695 mg/L, respectively. MTT results showed that the four extracts except IFra2 inhibited significantly proliferation of thyroid squamous cell carcinoma cells （sw579） at the concentration of 4.4 μg/mL and their inhibition rates increased in a time-dependant manner within 72 hours. Inhibition rates of IPTal and IPTel had a positive correlation with time and concentration at range of 0.3125-5 μg/mL and the highest inhibition rate was up to 90%.