文献类型：期刊文献

英文题名：Sensitive Fluorescent Sensor for Recognition of HIV-1 dsDNA by Using Glucose Oxidase and Triplex DNA

作者：Li, Yubin[1];Liu, Sheng[1];Ling, Liansheng[2]

机构：[1]Guangdong Ocean Univ, Coll Chem & Environm, Zhanjiang 524088, Peoples R China;[2]Sun Yat Sen Univ, Sch Chem, Guangzhou 510275, Guangdong, Peoples R China

年份：2018

卷号：2018

外文期刊名：JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY

收录：SCI-EXPANDED(收录号：WOS:000430216400001)、、Scopus(收录号：2-s2.0-85045742428)、WOS

基金：This work was supported by the Natural Science Foundation of Guangdong Ocean University (no. C16401) and Doctoral Initiating Project of Guangdong Ocean University Foundation for Natural Sciences (no. R17013).

语种：英文

外文摘要：A sensitive fluorescent sensor for sequence-specific recognition of double-stranded DNA (dsDNA) was developed on the surface of silver-coated glass slide (SCGS). Oligonucleotide-1 (Oligo-1) was designed to assemble on the surface of SCGS and act as capture DNA, and oligonucleotide-2 (Oligo-2) was designed as signal DNA. Upon addition of target HIV-1 dsDNA (Oligo-3 center dot Oligo-4), signal DNA could bind on the surface of silver-coated glass because of the formation of C center dot GoC in parallel triplex DNA structure. Biotin-labeled glucose oxidase (biotin-GOx) could bind to signal DNA through the specific interaction of biotin-streptavidin, thereby GOx was attached to the surface of SCGS, which was dependent on the concentration of target HIV-1 dsDNA. GOx could catalyze the oxidation of glucose and yield H2O2, and the HPPA can be oxidized into a fluorescent product in the presence of HRP. Therefore, the concentration of target HIV-1 dsDNA could be estimated with fluorescence intensity. Under the optimum conditions, the fluorescence intensity was proportional to the concentration of target HIV-1 dsDNA over the range of 10 pM to 1000 pM, the detection limit was 3 pM. Moreover, the sensor had good sequence selectivity and practicability and might be applied for the diagnosis of HIV disease in the future.