文献类型：期刊文献

英文题名：Accumulation and depuration of paralytic shellfish poisoning toxins in the oyster Ostrea rivularis Gould - Chitosan facilitates the toxin depuration

作者：Xie, Wancui[1];Liu, Xiaoli[2];Yang, Xihong[1];Zhang, Chaohua[1];Bian, Zhongyuan[1]

机构：[1]Guangdong Ocean Univ, Sch Food Sci & Technol, Zhanjiang 524088, Peoples R China;[2]Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214036, Peoples R China

年份：2013

卷号：30

期号：2

起止页码：446

外文期刊名：FOOD CONTROL

收录：SCI-EXPANDED(收录号：WOS:000313535500014)、、Scopus(收录号：2-s2.0-84867616195)、WOS

基金：The present research was financially supported by the Modern Agro-industry Technology Research System of China and the Project 31271938 supported by National Natural Science Foundation of China. Prof. Tianjiu Jiang from the Institute of Hydrobiology Research Center of Jinan University is gratefully acknowledged for his technical assistance.

语种：英文

外文关键词：High-performance liquid chromatography-fluorescence detection systems (HPLC-FLD); Mouse bioassay (MBA); Chitosan (CTS); Ostrea rivularis Gould (ORG); Paralytic shellfish poisoning (PSP) toxins; Accumulation; Depuration

外文摘要：The accumulation and depuration of paralytic shellfish poisoning (PSP) toxins in the oyster of Ostrea rivularis Gould (ORG) were investigated in this study. Firstly, PSP toxin-accumulating in ORG were produced by feeding with the toxic algae Alexandrium minutum cultured in the laboratory. The feeding amounts were designed for three concentrations of 4.0 x 10(3), 8.0 x 10(3), and 1.2 X 10(4) cells/mL. After 20 day's feeding, the toxicity in the ORG became high levels of 28.86, 31.17, and 38.98 MU/g, respectively. And the toxin-accumulating ORG fed only for 2 d with 8.0 x 10(3) cells/mL which toxicity was 11.93 MU/g were selected for deputation experiment. In the deputation process, the ORG were fed with nontoxic microalgae (MA) of Chlorella spp., chitosan (CTS), and MA + CTS. Feeding with 8.0 x 10(3) cells/mL MA and 0.05 g/L CTS resulted in the largest PSP toxin reduction in the ORG. After 7 d of depuration feeding, the toxicity of the PSP toxin decreased from 11.93 to 3.47, 1.40, and 0.50 MU/g, as determined by the mouse bioassay (MBA). High performance liquid chromatography - fluorescence detection systems (HPLC-FLD) was applied in the qualitative and quantitative detection of PSP toxins in the ORG during accumulation and depuration. Chromatograms of samples not containing oxidation and acidulating reagents in the mobile phase were used to identify unknown peaks. The HPLC results showed that PSP toxicity before depuration was 9.07 MU/g. After 7 d of depuration, feeding by CTS and MA + CTS was reduced to 1.41 and 0.12 MU/g, respectively. The laboratory experiments showed that CTS had a remarkable effect on PSP removal in ORG. The toxicity and toxin profiles of ORG were investigated by MBA combined with post-column HPLC-FLD. The results of both methods supplemented each other, thereby elucidating the accumulation, deputation, and transformation of PSP toxins from the toxic algae in ORG. (C) 2012 Elsevier Ltd. All rights reserved.