文献类型：期刊文献

英文题名：Molecular cloning and characterisation of scavenger receptor class B in pearl oyster Pinctada fuctada martensii

作者：Lei, Chao[1];Hao, Ruijuan[1];Zheng, Zhe[1];Deng, Yuewen[1,2];Wang, Qingheng[1,2];Li, Junhui[1]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China;[2]Pearl Breeding & Proc Engn Technol Res Ctr Guangd, Zhanjiang 524088, Peoples R China

年份：2017

卷号：30

起止页码：12

外文期刊名：ELECTRONIC JOURNAL OF BIOTECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000416721500004)、、EI(收录号：20174704415506)、Scopus(收录号：2-s2.0-85034088263)、WOS

基金：The study was financially supported by Graduate Education Innovation Program of Guangdong Ocean University (201721), Administration of Ocean and Fisheries of Guangdong Province (B201601Z09) and Innovation Strong School Project of Guangdong Province (2014GKXM049).

语种：英文

外文关键词：Carotenoids; cDNA; Gene expression; Hydrophobic pigments; Isoprenoid pigments; Marine bivalve molluscs; Precursor of abscisic acid; Precursor of phytohormones; RNA extraction; Saltwater oysters; Vitamin a

外文摘要：Background: Molluscs can accumulate carotenoids in their body tissues by predominantly feeding on aquatic plant sources. Carotenoid transport and absorption are determined by the regulation of various proteins such as Scavenger receptor class B(SR-BI). We report the identification and characterisation of pearl oyster Pinctada fuctada martensii SR-BI (PmSR-BI). The correlation between total carotenoid content (TCC) and gene expression was also estimated. Results: The full-length cDNA of PmSR-BI was 1828 bp, including an open-reading frame encoding of 1518 bp with a pI value of 5.83. PmSR-BI protein contains a hydrophobic CD36 domain and four centrally clustered cysteine residues for the arrangement of disulphide bridges. The deduced amino acid sequence had an identity of 30% to 60% with the SR-B of other organisms. Reverse transcription polymerase chain reaction analysis showed that mRNA transcripts were expressed in multiple tissues of adult pearl oyster. A higher expression of PmSR-BI gene was observed in the hepatopancreas than in the adductor muscle, gill and mantle. The TCC and gene expression of PmSR-BI were significantly correlated (P < 0.05), with a correlation coefficient of 0.978. Conclusions: The results suggested that PmSR-BI is involved in the absorption of carotenoids in the pearl oyster P. fuctada martensii. (C) 2017 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved.