文献类型：期刊文献

英文题名：Layer-by-layer multienzyme assembly for highly sensitive electrochemical immunoassay based on tyramine signal amplification strategy

作者：Zhou, Jun[1,2,3];Tang, Juan[1,2];Chen, Guonan[1,2];Tang, Dianping[1,2]

机构：[1]Fuzhou Univ, Minist Educ, Fuzhou 350108, Peoples R China;[2]Fuzhou Univ, Dept Chem, Fujian Prov Key Lab Anal & Detect Food Safety, Fuzhou 350108, Peoples R China;[3]Guangdong Ocean Univ, Modern Biochem Ctr, Zhanjiang 524088, Peoples R China

年份：2014

卷号：54

起止页码：323

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：SCI-EXPANDED(收录号：WOS:000333071500051)、、EI(收录号：20134917051578)、Scopus(收录号：2-s2.0-84888409942)、WOS

基金：Support by the "973" National Basic Research Program of China (2010CB732403), the Research Fund for the National Science Foundation of Fujian Province (2011J06003), the Doctoral Program of Higher Education of China (20103514120003), the National Natural Science Foundation of China (21075019 & 41176079), and the Program for Changjiang Scholars and Innovative Research Team in University (IRT1116) is gratefully acknowledged.

语种：英文

外文关键词：Electrochemical immunosensor; Nanosilver-doped bovine serum albumin microspheres; Tyramine signal amplification; Multienzyme assembly; Carcinoembryonic antigen

外文摘要：A new sandwich-type electrochemical immunosensor based on nanosilver-doped bovine serum albumin microspheres (Ag@BSA) with a high ratio of horseradish peroxidase (HRP) and detection antibody was developed for quantitative monitoring of biomarkers (carcinoembryonic antigen, CEA, used in this case) by coupling enzymatic biocatalytic precipitation with tyramine signal amplification strategy on capture antibody-modified glassy carbon electrode. Two immunosensing protocols (with and without tyramine signal amplification) were also investigated for the detection of CEA and improved analytical features were acquired with tyramine signal amplification strategy. With the labeling method, the performance and factors influencing the electrochemical immunoassay were studied and evaluated in detail. Under the optimal conditions, the electrochemical immunosensor exhibited a wide dynamic range of 0.005-80 ng mL(-1) toward CEA standards with a low detection limit of 5.0 pg mL(-1). Intra- and inter-assay coefficients of variation were below 11%. No significant differences at the 0.05 significance level were encountered in the analysis of 6 clinical serum specimens and 6 spiked new-born cattle serum samples between the electrochemical immunoassay and the commercialized electrochemiluminescent immunoassay method for the detection of CEA. (C) 2013 Elsevier B.V. All rights reserved.