文献类型：期刊文献

英文题名：Evolution and function analysis of interleukin-17 gene from Pinctada fucata martensii

作者：Cao, Yanfei[1];Yang, Shuai[1];Feng, Chuzhang[1];Zhan, Waner[1];Zheng, Zhe[1];Wang, Qingheng[1];Deng, Yuewen[1];Jiao, Yu[1];Du, Xiaodong[1]

机构：[1]Guangdong Ocean Univ, Fishery Coll, Zhanjiang 524025, Peoples R China

年份：2019

卷号：88

起止页码：102

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000465060500012)、、WOS

基金：The studies were financially supported by grants of the National Natural Science Foundation of China (grant numbers 31672626), the Guangdong Marine and Fishery Bureau (grant numbers Z2015004), Excellent Young Scientist Foundation of Guangdong Province (grant numbers 2015090), Graduate education innovation program of Guangdong Ocean University (grant numbers 201839), Project of Enhancing School with Innovation of Guangdong Ocean University (grant numbers GDOU2016050249).

语种：英文

外文关键词：Interleukin-17; Immune; Pinctada fucata martensii; Mollusk; Genomic analysis

外文摘要：Interleukin-17 (IL-17) is a proinflammatory cytokine that plays an important role in immune responses. In this study, we identified 57 IL-17 genes from the genomes of six marine invertebrates, including Pinctada fucata martensd, Crassostrea gigas, Lottia gigantea, Capitella teleta, Mizuhopecten yessoensis, and Mytilus galloprovincialis. Phylogenetic analysis showed that all invertebrate IL-17 genes were clustered into one group, implying that invertebrate IL-17 evolved from one common ancestral gene. From the extron-intron analysis, we found many intronless IL-17 genes in mollusks, which may be caused by retroposition. Tissue and development transcriptomic analysis showed that the expression of PmIL-17 was tissue and developmental stage-specific. Moreover, we cloned the full length of the IL-17-2 gene from P. f. martensii (PmIL-17-2) and explored its function in the immune response. The full-length cDNA of PmIL-17-2 is 719 bp, containing an open reading frame of 564 bp, a 5'-untranslated region (UTR) of 31 bp, and a 3'-UTR of 124 bp with a 30 bp poly (A) tail. PmIL-17-2 had a strong response to lipopolysaccharide (LPS), indicating that the PmIL-17.2 participates in innate immune responses. In situ hybridization of hemocytes showed that PmIL-17-2 was mainly produced by granulosa cells, and the number of the stained granulosa increased after LPS stimulation. These results lay the foundation for the research of IL-17 family in marine invertebrates.