文献类型：期刊文献

中文题名：玉屏风多糖对草鱼肠黏膜形态结构及主要免疫与吸收相关基因表达的影响

英文题名：Effects of Yupingfeng Polysaccharide on Morphology of Intestinal Mucosa and Expression of Main Immune and Absorption Genes of Grass Carp( Ctenopharyngodon idellus)

作者：尹福泉[1];吴勇亮[2];刘明珠[1,3];谭淑雯[2];杨映[2];于辉[2]

机构：[1]广东海洋大学农学院,湛江524088;[2]佛山科学技术学院生命科学与工程学院,佛山528231;[3]广州中国科学院先进技术研究所,广州511458

年份：2017

卷号：29

期号：10

起止页码：3718

中文期刊名：动物营养学报

外文期刊名：CHINESE JOURNAL OF ANIMAL NUTRITION

收录：CSTPCD、、北大核心2014、CSCD2017_2018、北大核心、CSCD

基金：国家科技计划项目(2015GA780004);佛山市科技创新专项资金(2013AG10005);广东海洋大学创新强校项目(GDOU2016050208);广东省草鱼疾病防控工程技术研究中心建设项目[粤科函产学研字[2016]176号;粤科函产学研字[2015]1487号]

语种：中文

中文关键词：草鱼;玉屏风多糖;肠道绒毛;白介素-2;干扰素y;钠葡萄糖转运蛋白1;葡萄糖转运蛋白2

外文关键词：grass carp;;Yupingfeng polysaccharide;;intestinal villus;;IL-2;;IFN-γ;;SGLT-1;;GLUT-2

中文摘要：本试验旨在探讨不同比例玉屏风多糖对草鱼肠黏膜形态结构及主要免疫与吸收相关基因表达的影响。试验选择750尾平均体重为(74.50±2.50)g的健康草鱼,随机分为5组,每组6个重复,每个重复25尾。对照组(Ⅰ组)投喂基础饲料,试验组(Ⅱ~Ⅴ组)投喂在基础饲料基础上分别添加0.8、1.2、1.6、2.0 g/kg玉屏风多糖的试验饲料。预试期7 d,正试期28 d。结果表明:1)与对照组相比,在试验第14天时,Ⅴ组的隐窝深度显著降低(P<0.05),而绒腺比则显著提高(P<0.05)。2)对照组相比,在试验第7天时,Ⅲ和Ⅳ组头肾中白介素-2(IL-2)mRNA相对表达量显著或极显著提高(P<0.05或P<0.01),Ⅱ、Ⅲ、Ⅳ和Ⅴ组头肾中干扰素γ(IFN-γ)mRNA相对表达量均极显著提高(P<0.01),Ⅲ、Ⅳ和Ⅴ组肠道中钠葡萄糖转运蛋白1(SG LT-1)和葡萄糖转运蛋白2(GLUT-2)mRNA相对表达量显著或极显著提高(P<0.05或P<0.01))。3)与对照组相比,在试验第14天时,Ⅳ和Ⅴ组头肾中IL-2 mRNA相对表达量极显著提高(P<0.01),Ⅱ、Ⅲ、Ⅳ和Ⅴ组头肾中IFN-γmRNA相对表达量显著或极显著提高(P<0.05或P<0.01),Ⅲ、Ⅳ和Ⅴ组肠道中SG LT-1和G LUT-2 mRNA相对表达量极显著提高(P<0.01)。4)与对照组相比,在试验第21天时,Ⅳ组头肾中IL-2 mRNA相对表达量显著提高(P<0.05),Ⅱ、Ⅲ、Ⅳ和Ⅴ组头肾中IFN-γmRNA相对表达量极显著提高(P<0.01),Ⅱ、Ⅲ、Ⅳ和Ⅴ组肠道中SG LT-1和G LUT-2 mRNA相对表达量显著或极显著提高(P<0.05或P<0.01)。5)与对照组相比,在试验第28天时,Ⅳ和Ⅴ组头肾中IL-2 mRNA相对表达量极显著提高(P<0.01),Ⅲ、Ⅳ和Ⅴ组头肾中IFN-γmRNA相对表达量显著或极显著提高(P<0.05或P<0.01),Ⅱ、Ⅲ、Ⅳ和Ⅴ组肠道中SGLT-1 mRNA相对表达量显著或极显著提高(P<0.05或P<0.01)。综上所述,在草鱼饲料中添加一定量的玉屏风多糖能够改善肠黏膜形态结构,促进肠道中SGLT-1和GLUT-2基因的表达,调控头肾中IL-2和IFN-γ基因的表达,从而提高肠道吸收功能、增强机体免疫力。从节约饲养成本出发,草鱼饲料中玉屏风多糖最适添加量为1.6 g/kg。

外文摘要：This experiment w as designed to investigate the effects of Yupingfeng polysaccharide on morphology of intestinal mucosa and expression of main immune and absorption genes of grass carp( Ctenopharyngodon idellus). A total of 750 healthy grass carp w ith an average body w eight of( 74.50 ± 2.50) g w ere randomly assigned to 5 groups w ith 3 replicates per group and 50 fish per replicate. The first group( group Ⅰ) w as the control group and fed a basal diet,and the remaining four groups( groups Ⅱ,Ⅲ,Ⅳ and Ⅴ) w ere experimental groups and fed experimental diets supplementing 0.8,1.2,1.6 and 2.0 g/kg Yupingfeng polysaccharide based on the basal diet,respectively. The pre-trial period w as 7 days,and the formal trial period w as 28 days.The results show ed as follow s: 1) feeding for 14 days,the crypt depth of group Ⅴ w as significantly low er than that of control group( P< 0. 05),and the villus height/crypt depth of group Ⅴ w as significantly higher than that of control group( P< 0.05). 2) On the trial day 7,compared w ith the control group,the relative expression level of interleukin-2( IL-2) mRNA in head-kidney of groups Ⅲ and Ⅳ w as significantly or extremely significantly increased( P< 0.05 or P< 0.01),the relative expression level of interferon γ( IFN-γ) mRNA in head-kidney of groups Ⅱ,Ⅲ,Ⅳ and Ⅴ w as extremely significantly increased( P< 0.01),and the relative expression levels of sodium glucose transporter 1( SG LT-1) and glucose transport protein 2( G LUT-2) mRNA in intestine of groups Ⅲ,Ⅳ and Ⅴ w ere significantly or extremely significantly increased( P < 0. 05 or P <0.01). 3) On the trial day 14,compared w ith the control group,the relative expression level of IL-2 mRNA in head-kidney of groups Ⅳ and Ⅴ w as extremely significantly increased( P< 0.01),the relative expression level of IFN-γ mRNA in head-kidney of groups Ⅱ,Ⅲ,Ⅳ and Ⅴ w as significantly or extremely significantly increased( P< 0.05 or P< 0.01),and the relative expression levels of SG LT-1 and G LUT-2 mRNA in intestine of groups Ⅲ,Ⅳ and Ⅴ w ere extremely significantly increased( P< 0.01). 4) On the trial day 21,compared w ith the control group,the relative expression level of IL-2 mRNA in head-kidney of group Ⅳ w as significantly increased( P< 0.05),the relative expression level of IFN-γ mRNA in head-kidney of groups Ⅱ,Ⅲ,Ⅳand Ⅴ w as extremely significantly increased( P < 0. 01),and the relative expression levels of SG LT-1 and G LUT-2 mRNA in intestine of groups Ⅱ,Ⅲ,Ⅳ and Ⅴ w ere significantly or extremely significantly increased( P<0.05 or P<0.01). 5) On the trial day 28,compared with the control group,the relative expression level of IL-2 mRNA in head-kidney of groups Ⅳ and Ⅴ w as extremely significantly increased( P< 0.01),the relative expression level of IFN-γ mRNA in head-kidney of groups Ⅲ,Ⅳ and Ⅴ w as significantly or extremely significantly increased( P< 0.05 or P< 0.01),and the relative expression levels of SG LT-1 and G LUT-2 mRNA in intestine of groups Ⅱ,Ⅲ,Ⅳ and Ⅴ w ere significantly or extremely significantly increased( P< 0.05 or P<0. 01). On the w hole,supplementing a certain amount of Yupingfeng polysaccharide in the diet can improve the morphology of intestinal mucosa,promote the expression of SG LT-1 and G LUT-2 genes in intestine,regulate the expression of IL-2 and IFN-γ genes in head-kidney,and then improve the intestinal absorption function and immunity of grass carp. In consideration of saving cost,the optimum supplemental level of Yupingfeng polysaccharide in the diet of grass carp is 1. 6 g/kg.[Chinese Journal of Animal Nutrition,2017,29( 10) : 3718-3725]