文献类型：期刊文献

中文题名：鱼源溶藻弧菌胞外产物的特性研究

英文题名：CHARACTERIZATION OF EXTRACELLULAR PRODUCTS FROM VIBRIO ALGINOLYTICUS ISOLATED FROM MARICULTURED FISH

作者：左凤琴[1];简纪常[1];吴灶和[1]

机构：[1]广东海洋大学水产学院广东省水产经济动物病原生物学及流行病学重点实验室

年份：2006

期号：5

起止页码：553

中文期刊名：水生生物学报

外文期刊名：Acta Hydrobiologica Sinica

收录：CSTPCD、、北大核心2004、CSCD2011_2012、北大核心、CSCD

基金：广东省自然基金项目(011812);广东省科技计划项目(2001A305030201);教育部重点项目(204108)

语种：中文

中文关键词：溶藻弧菌;胞外产物;特性

外文关键词：Vibrio alginolyticus; Extracellular product; Character;

中文摘要：溶藻弧菌经胰胨大豆胨琼脂培养基(TSA+2%NaCl)培养48h后,培养物用PBS洗脱、离心,再经孔径0.22μm微孔滤膜过滤,制得胞外产物(Extracellular product,ECP)。经检测,该ECP具有淀粉酶、酪蛋白酶、卵磷脂酶和脂肪酶等4种酶活性,其中淀粉酶的活性最高,酪蛋白酶、脂肪酶活性次之,卵磷脂酶活性较低,未检测出明胶酶及脲酶活性;能溶解断斑石鲈(Pomadasys hasta)、眼斑拟石首鱼(Sciaenops ocellatus)和赤点石斑鱼(Epinephelus akaara)的红细胞,而不能溶解鸡、羊及O型人红细胞;对小白鼠和石斑鱼的LD50分别为0.08mg/g和0.17mg/g,95%可信限分别为0.06—0.12mg/g和0.07—0.28mg/g;50mmol/L EDTA和100mmol/L苯甲基磺酰氟能使胞外蛋白酶(Extracellular pro-teinase,ECPase)的活性分别下降83.40%和51.32%;ECPase对热稳定性较差,碱性条件下活性较高,其最适温度为50℃,最适pH为8.0。

外文摘要：Vibrio alginolyticus is an opportunist pathogen,which exists extensively in seawater,and causes diarrhea in human and vibriosis in maricultured animals.Fewer studies on the extracellular product(ECP) of V.alginolyticus were reported.In our experiments the ECP was extracted from V.alginolyticus and the biological and physicochemical characters of the ECP were assessed.After V.alginolyticus had grown on tryptic soy agar(TSA) supplemented with 2%NaCl for 48h,the bacteria on the Petri dish were washed away by P...