文献类型：期刊文献

英文题名：Phoenixin participated in regulation of food intake and growth in spotted scat, Scatophagus argus

作者：Wang, Mei[1];Deng, Si-Ping[1];Chen, Hua-Pu[1,2];Jiang, Dong-neng[1];Tian, Chang-Xu[1];Yang, Wei[1];Wu, Tian-li[1];Zhu, Chun-Hua[1];Zhang, Yong[3];Li, Guang-Li[1]

机构：[1]Guangdong Ocean Univ, Key Lab Marine Ecol & Aquaculture Environm Zhanji, Fisheries Coll, Guangdong Res Ctr Reprod Control & Breeding Techn, Zhanjiang 524088, Peoples R China;[2]Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China;[3]Sun Yat Sen Univ, Sch Life Sci, Guangdong Prov Engn Technol Res Ctr Hlth Breeding, State Key Lab Biocontrol,Guangdong Prov Key Lab A, Guangzhou 510275, Guangdong, Peoples R China

年份：2018

卷号：226

起止页码：36

外文期刊名：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000447114400005)、、Scopus(收录号：2-s2.0-85051815031)、WOS

基金：This study was supported by the Natural Science Foundation of Guangdong Province [2016A030313743, 2017A030313101; 20178090904022]; Key Research and Development Plan of Hainan Province [ZDYF2018225]; Marine Fishery Science and Technology Extension Projects of Guangdong [A201608B01, 2017A00121; Zhanjiang Science and Technology Bureau [2016A03017] and Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology (No. 2016LMFS-B12).

语种：英文

外文关键词：Scatophagusargus; Phoenixin-14; Phoenixin-20; Food intake; Growth

外文摘要：Phoenixin (Pnx) is an endogenous peptide known to be involved in reproduction and food intake in rats, with two active isoforms, phoenixin-14 (Pnx-14) and phoenixin-20 (Pnx-20). However, little is known about the functions of Pnx in teleost. Here, pnx was cloned and was detected in all tissues of both male and female in spotted scat (Scatophagus argus), including growth axis, hypothalamus, pituitary, and liver. Real-time PCR analysis showed that pnx in the hypothalamus increased significantly after 2 d and 7 d fasting, while reduced significantly after re-feeding (P < 0.05). When pituitary and liver fragments were cultured in vitro with Pnx-14 and Pnx-20 (10 nM and 100 nM) for 6 h, the expression of ghrh1 (growth hormone-releasing hormone receptor) and gh (growth hormone) in the pituitary, and ghrl (growth hormone receptor 1) in the liver increased significantly, except ghr2 (growth hormone receptor 2) incubated with 10 nM and 100 nM Pnx-20 and ghrl incubated with 10 nM Pnx-20. Similarly, the expression of ghrhr and gh in the pituitary, as well as ghrl and ghr2 in the liver, increased significantly after injecting S. argus with Pnx-14 and Pnx-20 (10 ng/g and 100 ng/g body weight). These results indicate that Pnx is likely to be involved in the regulation of food intake, and also regulates the growth of S. argus by increasing ghrhr and gh expression in the pituitary, ghrl and ghr2 in the liver, and ghrl directly in the liver.