文献类型：期刊文献

英文题名：Cytotoxicity of T-2 and modified T-2 toxins: induction of JAK/STAT pathway in RAW264.7 cells by hepatopancreas and muscle extracts of shrimp fed with T-2 toxin

作者：Wang, Xing[1];Wang, Yaling[1];Qiu, Mei[1,2];Sun, Lijun[1];Wang, Xiaobo[1];Li, Caihong[3];Xu, Defeng[1];Gooneratne, Ravi[4,5]

机构：[1]Guangdong Ocean Univ, College Food Sci & Technol, Guangdong Prov Key Lab Aquat Product Proc & Safet, Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Zhanjiang 524088, Peoples R China;[2]Nat Marine Prod Qual Supervis Inspect Ctr, Zhanjiang 524000, Peoples R China;[3]Guangdong Med Univ, Inst Biochem & Mol Biol, Dongguan 523808, Peoples R China;[4]Lincoln Univ, Ctr Food Res, POB 85084, Lincoln 7647, New Zealand;[5]Lincoln Univ, Innovat Ctr Food Res & Innovat, POB 85084, Lincoln 7647, New Zealand

年份：2017

卷号：6

期号：2

起止页码：144

外文期刊名：TOXICOLOGY RESEARCH

收录：SCI-EXPANDED(收录号：WOS:000396138500003)、、Scopus(收录号：2-s2.0-85014815215)、WOS

基金：This study was financially supported by the National Natural Science Foundation of China (NSFC No. 31171634 and No. 31371777), the Science and Technology Planning Project of Guangdong Province (No. 2014B020205006), a Guangdong Oceanic & Fishery Administration project (Guangdong Fiscal Government on Agriculture [2015] No. 115), and the Project of Enhancing School with Innovation of Guangdong Ocean University (No. 2016050205 and No. 2014050203 and GDOU2016050312).

语种：英文

外文摘要：T-2 can be biotransformed in animal tissues to modified T-2s (mT-2s). Food contaminated with T-2 and/or mT-2s is a hazard to both animals and humans, including the immune system. In this study, Litopenaeus vannamei were fed T-2 orally for 20 d, and hepatopancreas and muscle extracts, T-2, and T-2-glucuronide (T-2-GluA) were added to RAW264.7 in vitro and their effects on the JAK/STAT pathway were examined. STAT2 mRNA gene expression induced by hepatopancreas and muscle extracts was markedly higher compared with that of T-2 or T-2-GluA group. SCOSs, IL-6 and IL-1 beta mRNA gene expressions induced by hepatopancreas extract were greater than those induced by muscle extract. Muscle extract significantly activated STAT3 phosphorylation but inhibited STAT1 phosphorylation. Activation of the JAK/STAT pathway by hepatopancreas mT-2s was significantly higher than that by muscle extracts. Muscle and hepatopancreas extracts and T-2 also significantly induced IL-6 mRNA gene expression. With reference to phosphorylation levels, significant activation of JAK1 and STAT2 occurred with T-2 and JAK3 by muscle extract, JAK2 by hepatopancreas extract and STAT1 by T-2-GluA. This study showed that both T-2 and mT-2s are cytotoxic but the activation of the JAK/STAT pathway in RAW264.7 cells by T-2 was greater than that by mT-2s in hepatopancreas and muscle extracts from T-2-fed Litopenaeus vannamei.