文献类型：期刊文献

英文题名：Multiplex Species-Specific PCR Identification of Native Onchidiid Slugs in the South China Sea: A Useful Tool for Application in Onchidiid Slug Stock Management and Germplasm Conservation

作者：Huang, Jingyue[1,2,3];Ma, Haitao[2,3,4,5,6];Duan, Xixi[1,2,3];Wei, Zonglu[1,2,3];Zhang, Yinjie[1,2,3];Zhang, Tao[2,3,4,5];Chen, Xiaoqun[2,3,4,5];Qin, Yanping[2,3,4,5,6];Li, Jun[2,3,4,5,6];Yu, Ziniu[2,3,4,5,6];Pan, Ying[1];Zhang, Yuehuan[2,3,4,5,6]

机构：[1]Guangxi Univ, Coll Anim Sci & Technol, Key Lab Aquat Hlth Breeding & Nutr Regulat Guangxi, Nanning 530004, Peoples R China;[2]Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Key Lab Trop Marine Bioresources & Ecol, South China Sea Inst Oceanol,Guangdong Prov Key La, Guangzhou 510301, Peoples R China;[3]Chinese Acad Sci, Daya Bay Marine Biol Res Stn, Shenzhen 518124, Peoples R China;[4]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 518120, Peoples R China;[5]Agrotech Extens Ctr Guangdong Prov, Guangzhou 510145, Peoples R China;[6]Chinese Acad Sci, Hainan Prov Key Lab Trop Marine Biol Technol, Marine Ecoenvironm Engn Res Inst, Trop Marine Biol Res Stn Hainan, Sanya 572024, Peoples R China

年份：2026

卷号：16

期号：4

外文期刊名：ANIMALS

收录：SCI-EXPANDED(收录号：WOS:001699832000001)、、WOS

基金：This research was supported by Guangdong Science and Technology Plan Program (2024B1212050006); Guangxi Natural Science Foundation General Program Project (2025GXNSFAA069872); Natural Science Foundation of China Project (32260914); National Key Research and Development Program of China (2023YFD2401704; 2022YFC3103603; 2021YFC3100504); Hainan Provincial Key R&D Programme (ZDYF2024XDNY175); Shenzhen Basic Research Project (JCYJ20240813111707010); Guangzhou Science and Technology Project (202206010133; 2023B03J00165; 2024B03J1226); Guangdong Basic and Applied Basic Research Foundation (2023A1515010944; 2022A1515010203); Research on breeding technology of candidate species for Guangdong modern marine ranching (2024-MRB-00-001); National Marine Genetic Resource Center; the earmarked fund for CARS-49; Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) (No. SML2023SP234) and the Science and Technology Planning Project of Guangdong Province, China (2023B1212060047).

语种：英文

外文关键词：species identification; COI; Onchidium stuxbergi; Peronia verruculata; Onchidium reevesii; Platevindex martensi; Paromoionchis tumidus

外文摘要：To address the need for accurate onchidiid slug species identification, the present study engineered a multiplex PCR-based assay to facilitate the efficient, economical, and discrimination of five morphologically similar onchidiid slug species endemic to the South China Sea: Onchidium stuxbergi, Peronia verruculata, Onchidium reevesii, Platevindex martensi and Paromoionchis tumidus. A diagnostic multiplex PCR assay was designed based on a partial fragment of the mitochondrial cytochrome oxidase subunit I. It utilizes a shared forward primer and five taxon-specific reverse primers to simultaneously produce amplicons of varying sizes, each indicative of a particular species. Five species of Onchidiidae can be identified in a single reaction, which provides technical support for molecular identification of Onchidiidae. The development of a simple, effective and rapid multiple PCR identification method is of great significance for stock management and germplasm conservation of Onchidiidae.