文献类型：期刊文献

英文题名：Effect of dietary vitamin C on the growth performance and innate immunity of juvenile cobia (Rachycentron canadum)

作者：Zhou, Qicun[1];Wang, Ligai[2];Wang, Hualang[3];Xie, Fengjun[1];Wang, Tuo[3]

机构：[1]Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China;[2]Guangdong Ocean Univ, Coll Aqauculture, Zhanjiang 524025, Peoples R China;[3]Guangdong Evergreen Feed Ind Co Ltd, Aqua Feed Res & Dev Ctr, Zhanjiang 524094, Peoples R China

年份：2012

卷号：32

期号：6

起止页码：969

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000304511800003)、、Scopus(收录号：2-s2.0-84862804791)、WOS

基金：This study was funded by a fundamental research grant from the National Key Technologies R & D Program (Grant No: 2007BAD29B04) and also sponsored by K. C. Wong Magna Fund in NingboUniversity.

语种：英文

外文关键词：Rachycentron canadum; Vitamin C; Growth; Non-specific immunity; Hematologic parameter

外文摘要：This study was conducted to evaluate the effects of dietary vitamin C on growth performance, hematologic parameters and innate immune responses in juvenile cobia, Rachycentron canadum. Seven practical diets were formulated to contain 0.0 (as the basal diet), 13.6, 27.2, 54.4, 96.6, 193.4 and 386.5 mg ascorbic acid equivalent kg(-1) diet. Each diet was fed to triplicate groups of juvenile cobia with initial body weight of 5.5 g in 500-L cylindrical fiberglass tank. The results of 8 weeks feeding trial showed that typical vitamin C-deficient signs such as spinal deformation and body nigrescence were observed in the fish fed the basal diet. Fish fed the basal diet had significantly lower weight gain, specific growth rate (SGR), protein efficiency ratio (PER) and feed efficiency (FE) than those fed the diets supplemented with vitamin C. but no significant differences were observed among diets supplemented with vitamin C. However, survival rate was significantly affected by the dietary vitamin C levels, fish fed the basal diet had lower survival rate than those fed the diets supplemented with vitamin C. The ascorbic acid concentration in liver was correlated positively with the dietary vitamin C levels, however, the thiobarbituric acid reactive substances (TBARS) concentrations in liver was not significantly affected by the dietary vitamin C levels, although, fish fed the basal diet had the highest TBARS values among all treatments. The activities of serum lysozyme, superoxide dismutase (SOD), alkaline phophatase (AKP) and total immunoglobulin (Ig) were significantly influenced by the dietary vitamin C levels, fish fed the basal diet had lower lysozyme, SOD, AKP and total Ig than those fed diets supplemented with vitamin C. The serum glucose and triglyceride concentrations were significantly affected by the dietary vitamin C levels. Fish fed the basal diet had lower red blood cell and hemoglobin values than those fed the vitamin C supplemented diets. The challenge experiment with Vibrio harveyi showed that lower cumulative survival was in fish fed the unsupplemented diet, the cumulative survival were significantly increased with increase of the dietary ascorbic acid levels from 13.6 to 96.6 mg kg(-1), while the cumulative survival reached plateau when dietary ascorbic acid levels increased from 96.6 to 386.5 mg kg(-1). These results indicated that dietary vitamin C did significantly influence on growth performance and immune response of juvenile cobia. (C) 2012 Elsevier Ltd. All rights reserved.