文献类型：期刊文献

中文题名：液相色谱法检测鱼类肝脏中天然维生素A

英文题名：Determination of natural vitamin A in fish liver by liquid chromatography

作者：黄文婷[1];曹文红[1,2,3,4,5,6,7];章超桦[1,2,3,4,5,6,7];秦小明[1,2,3,4,5,6,7];高加龙[1,2,3,4,5,6,7];郑惠娜[1,2,3,4,5,6,7]

机构：[1]广东海洋大学食品科技学院,广东湛江524088;[2]国家贝类加工技术研发分中心,广东湛江524088;[3]广东省水产品加工与安全重点实验室,广东湛江524088;[4]广东省海洋生物制品工程实验室,广东湛江524088;[5]广东省海洋食品工程技术研究中心,广东湛江524088;[6]水产品深加工广东普通高等学校重点实验室,广东湛江524088;[7]大连工业大学海洋食品精深加工关键技术省部共建协同创新中心,辽宁大连116034

年份：2022

卷号：47

期号：6

起止页码：141

中文期刊名：中国油脂

外文期刊名：China Oils and Fats

收录：CSTPCD、、CSCD2021_2022、北大核心、CSCD、北大核心2020

基金：国家重点研发计划课题(2018YFD0901105)。

语种：中文

中文关键词：鱼;肝脏;维生素A;提取方法;检测方法

外文关键词：fish;liver;vitamin A;extraction method;detection method

中文摘要：为了准确测定鱼类肝脏中维生素A(维生素A_(1)、维生素A_(2))含量,对样品预处理方法(水浴皂化法、室温皂化法、直接提取法)和检测方法(正相色谱法、反相色谱法)进行筛选,并应用于9种经济鱼类肝脏中维生素A的测定。结果表明:维生素A_(1)、维生素A_(2)标准品用反相色谱法检测分离效果良好,且在各自线性范围内线性关系良好,R^(2)均大于0.99;水浴皂化法维生素A含量显著高于室温皂化法及直接提取法(p<0.05),且维生素A_(1)平均回收率为104.52%,维生素A_(2)平均回收率为90.94%;在9种鱼中,除了淡水鱼乌鳢和大口黑鲈外,其他淡水、海水鱼类肝脏中维生素A总含量均达200μg/100 g以上,其中海水鱼龙趸石斑鱼肝脏中维生素A总含量最高,达到14413.78μg/100 g。水浴皂化法结合反相色谱法精密度良好,适用于鱼类肝脏中维生素A的分析测定。

外文摘要：In order to accurately determine the content of vitamin A(vitamin A_(1),vitamin A_(2))in fish liver,sample pretreatment methods(water bath saponification,room temperature saponification,direct extraction)and detection methods(normal phase chromatography,reversed phase chromatography)were screened and applied to the determination of vitamin A in the liver of nine economic fish species.The results showed that vitamin A_(1) and vitamin A_(2) were separated effectively by reversed phase chromatography and showed good linear relationship within their respective linear ranges(R^(2)>0.99);the content of vitamin A extracted by water bath saponification was significantly higher than that by room temperature saponification and direct extraction(p<0.05),and its average recoveries of vitamin A_(1) and vitamin A_(2) were 104.52%and 90.94%,respectively.Except for the freshwater snakehead and big mouth bass,the total content of vitamin A in the livers of other freshwater fishes and marine fishes was more than 200μg/100 g,and the total content of vitamin A in the liver of marine giant grouper was the highest,reaching 14413.78μg/100 g.The water bath saponification method combined with reversed phase chromatography has good precision and is suitable for the determination of vitamin A in fish liver.