文献类型：期刊文献

英文题名：Transcriptome and lipidome integration unveils key mechanisms constraining bivalve larval sensitivity in an acidifying sea

作者：Xu, Yang[1];Masanja, Fortunatus[1];Deng, Yuewen[1];Zhao, Liqiang[1,2,3]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang, Peoples R China;[2]Guangdong Ocean Univ, Guangdong Sci & Technol Innovat Ctr Marine Inverte, Zhanjiang, Peoples R China;[3]Guangdong Ocean Univ, Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang, Peoples R China

年份：2025

卷号：54

外文期刊名：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS

收录：SCI-EXPANDED(收录号：WOS:001430981000001)、、Scopus(收录号：2-s2.0-85217945495)、WOS

基金：The present study has been made possible by grants from the National Science Foundation of China (42076121, M-0163, 42211530423), the Department of Education of Guangdong Province (2022ZDZX4012), the China Scholarship Council (NO. 2023-21), the Guangdong Zhujiang Talents Program (2021QN02H665), and the program for Scientific Research Start-up Funds of Guangdong Ocean University.

语种：英文

外文关键词：Ocean acidification; Coastal areas; Bivalves; Ruditapes philippinarum; Initial shell formation

外文摘要：The intensity, frequencye and duration of seawater acidification in coastal seas have already surpassed projections for open oceans. Bivalve larvae are extremely sensitive to intensifying coastal seawater acidificaiton during their initial shell building, a critical period constraining recruitment success and population maintenance, but underlying mechanisms of larval shell formation sensitivity to acidification remain largely debated. Here, we performed an integrated analysis of the transcriptome and lipidome of trochophore of Ruditapes philippinarum to compare the core molecular responses involved in initial shell formation under ambient (pH 8.1), moderately (pH 7.7), and severely (pH 7.4) acidified conditions. Ocean acidification (OA) affected the ion transport efficiency by inhibiting gene expression of key ion transporters, thereby inhibiting initial shell formation, but the gene downregulation in the moderate exposure group was more significant. OA also induced major membrane lipid remodeling in larvae, which also significantly affected the ion transport efficiency. The TAG content of larvae which sustained the energy supply for active transport of calcification substrates and synthesis of organic matrix in the severe exposure group was significantly reduced. Overall, OA inhibited the formation of the initial larval shell, but different levels of OA had different inhibitory mechanisms on the initial larval shell formation, and the present study also further identified the role of lipids in initial shell formation, which can provide a theoretical basis for for a more accurate and comprehensive assessment of the impact of OA on bivalve calcification in an acidifying ocean.