文献类型：期刊文献

英文题名：Chicken GHR natural antisense transcript regulates GHR mRNA in LMH cells

作者：Zhang, Li[1,2];Lin, Shudai[1];An, Lilong[2];Ma, Jinge[1];Qiu, Fengfang[1];Jia, Rumin[2];Nie, Qinghua[1];Zhang, Dexiang[1];Luo, Qingbin[1];Li, Ting[2];Wang, Zhang[2];Zhang, Xiquan[1]

机构：[1]South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Key Lab Chicken Genet Breeding & Reprod, Minist Agr,Coll Anim Sci, Guangzhou 510642, Peoples R China;[2]Guangdong Ocean Univ, Coll Agr, Zhanjiang 524088, Peoples R China

年份：2016

卷号：7

期号：45

起止页码：73607

外文期刊名：ONCOTARGET

收录：SCI-EXPANDED(收录号：WOS:000387452100089)、、WOS

基金：The authors would like to thank Prof. Yunfeng Wang and Yan Zhao (Harbin Veterinary Research Institute, Chinese Academy of Agricultural Science, Heilongjiang, China) for donating us LMH cells. This work was supported by the Chinese Postdoctoral Science Foundation (2013T60808), the National Natural Science Foundation (31301958, 31672412), the Guangdong Provincial Natural Science Foundation (S2013010013382) and the China Agriculture Research System (CARS-42-G05).

语种：英文

外文关键词：chicken; GHR natural antisense transcript; GHR mRNA; liver; LMH cell

外文摘要：Growth hormone receptor (GHR) played key roles in human and animal growth. Both human laron type dwarfism and sex linked dwarf chicken were caused by the mutation of GHR gene. In this study, we identified an endogenously expressed long non-coding natural antisense transcript, GHR-AS, which overlapped with the GHR mRNA (GHR-S) in a tail to tail manner. Spatial and temporal expression analyses indicated that GHR-AS were highly expressed in chicken liver and displayed ascending with the development of chicken from E10 to 3 w of age. Interfering GHR-AS caused GHR-S decreasing, accompanied with increasing of the inactive gene indicator, H3K9me2, in the GHR-S promoter regions in LMH cells. RNase A experiment exhibited that GHR-AS and GHR-S can form double strand RNAs at the last exon of GHR gene in vivo and in vitro, which hinted they could act on each other via the region. In addition, the levels of GHR-S and GHR-AS can be affected by DNA methylation. Compared the normal chicken with the dwarfs, the negative correlation trends were showed between the GHR-S promoter methylation status and the GHR-AS levels. This is the first report of that GHR gene possessed natural antisense transcript and the results presented here further highlight the fine and complicated regulating mechanism of GHR gene in chicken development.