文献类型：期刊文献

英文题名：Immune response and protective efficacy of mannosylated polyethylenimine (PEI) as an antigen delivery vector, administered with a Streptococcus agalactiae DNA vaccine in Nile tilapia (Oreochromis niloticus)

作者：Chen, Xin-Jin[1];Wangkahart, Eakapol[3];Cai, Jia[1,2];Huang, Yu[1,2];Jian, Ji-chang[1,2];Wang, Bei[1,2]

机构：[1]Guangdong Ocean Univ, Guangdong Higher Educ Inst, Coll Fishery, Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab, Zhanjiang 524002, Peoples R China;[3]Mahasarakham Univ, Fac Technol, Div Fisheries, Appl Anim & Aquat Sci Res Unit,Lab Fish Immunol &, Kantarawichai 44150, Mahasarakham, Thailand

年份：2023

卷号：135

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000961295900001)、、WOS

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 32002426, U20A2065, 32073006) .

语种：英文

外文关键词：Immune response; Man-PEI; Antigen delivery vector; Antibody titer; Gene expression analysis; Histopathological examination

外文摘要：This study examined the effectiveness of a DNA vaccine for S. agalactiae that was delivered by mannose-based polyethyleneimine (Man-PEI). The results showed that Man-PEI/pcDNA-Sip stimulated a higher serum anti-body titer compared to control or other vaccine groups (p < 0.05). Additionally, it induced higher expression of immune-related genes, and increased activities of superoxide dismutase (SOD), acid phosphatase (ACP) and alkaline phosphatase (AKP). Furthermore, the Man-PEI/pcDNA-Sip group showed an improved relative percent survival (RPS) of 85.71%. These results demonstrate the potential value of Man-PEI as a vaccine delivery vehicle, and suggest that it can be effective in boosting the immune protective rate induced by pcDNA-Sip vaccines.