文献类型：期刊文献

英文题名：Male-specific Dmrt1 is a candidate sex determination gene in spotted scat (Scatophagus argus)

作者：Mustapha, Umar Farouk[1];Jiang, Dong-Neng[1];Liang, Zhi-Hua[1];Gu, Hao-Tian[1];Yang, Wei[1];Chen, Hua-Pu[1];Deng, Si-Ping[1];Wu, Tian-Li[1];Tian, Chang-Xu[1];Zhu, Chun-Hua[1];Li, Guang-Li[1]

机构：[1]Guangdong Ocean Univ, Fisheries Coll,Guangdong Res Ctr Reprod Control &, Guangdong Higher Educ Inst,Key Lab Aquaculture So, Key Lab Marine Ecol & Aquaculture Environm Zhanji, Zhanjiang 524088, Peoples R China

年份：2018

卷号：495

起止页码：351

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:000439123500041)、、Scopus(收录号：2-s2.0-85048534851)、WOS

基金：This study was supported by grants from NSFC of China (31702326 and 41706174), Department of education of Guangdong province (2016JGXM_ZD_45), Zhanjiang Science and Technology Bureau (2016A03017), Natural Science Foundation of Guangdong Province (2016A030313743 and 2017A030313101), Guangdong Ocean University Natural Science Research Program (2017), Project of Provincial Key Platform and Major Scientific Research of Colleges and Universities in Guangdong (2015KTSCX058), Sail Projects of Guangdong (2014.1) and Marine Fishery Science and Technology Extension Projects of Guangdong (A201408A06 and A201608B01). The authors cordially thank Prof. Jun-Hong Xia from the College of Life Sciences, Sun Yat-Sen University, Guangzhou, People's Republic of China for discussion and suggestion.

语种：英文

外文关键词：Sex determination gene; Fish; Dmrt1; Marker; Spotted scat (Scatophagus argus)

外文摘要：D The Scatophagus argus is a cultured marine teleost fish in which females grow faster and larger than males, therefore cloning the master sex-determination (SD) gene will be helpful for its sex control in aquaculture. A well-conserved Dmrt1 (doublesex-and mab-3-related transcription factor-1) gene, encoding zinc finger-like DNA binding motif (DM-domain) plays a critical role in sex determination and differentiation in vertebrates. Here, two pairs of sex-specific primers, Dmrt3-Marker-F/R and Dmrt1-Marker-F/R were developed from Dmrt3 and Dmrt1 transcripts in S. argus respectively. The single-nucleotide polymorphisms (SNP) at position - 151 and 166 bp on the DNA amplified by Dmrt3-Marker-F/R are all homozygous CC GG genotype in females and heterozygous CG GA genotype in the males. The Dmrt1-Marker-F/R could only amplify a 3.2 kb fragment in males. Both Markers were confirmed in 246 adult S. argus (113 males and 133 females) from five populations in China. These results indicate that the sexes of S. argus are controlled by sex chromosomes (XX females, XY males). The nucleotide at position 166 bp of the DNA amplified by Dmrt3-Marker-F/R is found to be a donor splice site mutation (c.400+ 1G > A) in Dmrt3 which generates a truncated isoform. The truncated Dmrt3 is linked to male sex and thereafter named as Dmrt3(Delta)-Y, and its allele is the normal Dmrt3 on the X chromosome. The DNA amplified by Dmrt1-Marker-F/R is a fragment between exon 2 and exon 3 of a normal Dmrt1 implying that Dmrt1 is male-specific and located on the Y chromosome. A truncated Dmrt1 (Dmrt1b) was found to exist in both males and females. The Dmrt1b is with high similarity with Dmrt1 indicating Dmrt1b is the allele of Dmrt1 located on the X chromosome. RT-PCR revealed that Dmrt1 is highly expressed in the testes, while it is not expressed in the ovaries. Dmrt3(Delta)-Y is only expressed weakly in some of the testes, while it is not expressed in the ovaries. The testicular specifically expressed Dmrt1 is the candidate SD gene on the Y chromosome in S. argus.